ResourceLife Sciences
Detection of rare cells and circulating tumor cells using flow cytometric methods
27 Jan 2021Circulating tumor cells (CTC) are rare cells shed from a solid tumor or metastases that circulate through the bloodstream. Detection of CTC poses a challenge because they are present at extremely low concentrations, as few as a couple CTC in several milliliters of blood, which contain up to 3 to 6 million cells per mL. To determine the sensitivity and accuracy of rare cell detection on the Agilent NovoCyte Quanteon flow cytometer, a tumor cell spiking assay was used in which a small number of cells from a tumor cell line was added to whole blood and detected by a cell surface stain.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Blood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.TumorsTumor research focuses on understanding abnormal cell growth that leads to cancer. Identifying biomarkers, studying tumor microenvironments, and developing targeted therapies are critical for advancing cancer treatment. Early detection and personalized treatment options are key to improving outcomes for patients. Browse our peer-reviewed product directory to explore tools for tumor research, diagnostics, and cancer therapies; compare products, read customer reviews, and get pricing directly from manufacturers.