Culture-independent molecular detection of pathogens

Molecular analysis of microbes directly from samples can be challenging as the contained human DNA compromises the sensitivity of broad-range assays. Molzym has introduced the Micro-Dx™, a unique CE IVD test for culture-free diagnosis of bacterial and fungal targets directly from samples. It includes an automated protocol for the depletion of human DNA prior to microbial DNA extraction and purification. For the detection of microbial DNA, 16S & 18S rDNA broad-range PCR assays are used, with a first result promised after only four hours. Sanger sequencing and BLAST analysis are applied to identify the pathogens on the species or genus level.