Characterizing protein oligomerization with automated mass photometry

In this application note, Refeyn discusses the importance of protein oligomerization and its role in protein function. Mass photometry is a powerful technique for studying protein oligomerization, as it provides high-resolution mass distributions of proteins in solution. Automation through Refeyn's TwoMP enhances the benefits of mass photometry, enabling rapid screening and analysis of oligomeric states. The experiments presented demonstrate the ability of mass photometry to characterize oligomerization behavior and the influence of factors like calcium concentration and inhibitor molecules.