ResourceLife Sciences
Cell culture quality control during T cell expansion
3 Aug 2019In this application note, Thermo Fisher Scientific describes a workflow that enables the isolation, activation, and expansion of robust T cells for downstream immuno-oncology applications. The Countess II FL Automated Cell Counter provides fast, accurate measurements of T cell viability and concentration.
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Invitrogen™ Countess™ 3 FL Automated Cell Counter
Thermo Fisher ScientificThe Countess 3 FL Automated Cell Counter is both a brightfield and fluorescence cell counter that uses advanced machine-learning image analysis algorithms to deliver accurate cell counts and viability. The fluorescence capabilities are user-determined with the ability to switch out light cubes and is useful for reporting and measuring fluorescent protein expression, apoptosis, and cell viability using fluorescent reagents.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.T Cells