Invitrogen™ Countess™ 3 FL Automated Cell Counter
The Countess 3 FL Automated Cell Counter is both a brightfield and fluorescence cell counter that uses advanced machine-learning image analysis algorithms to deliver accurate cell counts and viability. The fluorescence capabilities are user-determined with the ability to switch out light cubes and is useful for reporting and measuring fluorescent protein expression, apoptosis, and cell viability using fluorescent reagents.

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Great equipment and easy to work with
Cell counting
In cell culture, automated cell counting is quite useful as the cell counting is so rapidly done, that such instrument helps alot to reduce your time and allows you an idea of the total cells for further processing, subculture and experimentation. Previously, I was manually counting the cells but now this instrument has taken the place especially when I do multiple treatments for different cell types .
Review Date: 11 Dec 2024 | Thermo Fisher Scientific
The Countess 3 FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, fully automated focus and lighting capability, and image analysis software driven by a neural network-based machine-learning algorithm, for rapid assessment of primary and immortalized cell lines. The Countess 3 FL counter is capable of brightfield or fluorescence illumination with three-channel flexibility (brightfield and two optional fluorescence channels) enabling researchers to count cells, monitor fluorescent protein expression, gain insights to apoptotic processes, and measure cell viability.
- Accurate—eliminates the subjectivity of manual cell counting and user-to-user variability
- Flexible—accepts up to two EVOS light cubes, user selectable from more than a dozen fluorescent channels
- Fast—counts live and dead cells, measures viability, reports average cell size, and saves data with a single touch, in about 20 seconds
- Convenient—requires no cleaning or routine maintenance; high-resolution capacitive touchscreen and simple user interface provide quick startup and requires minimal training
The Countess 3 FL Automated Cell Counter works in two simple steps:
Pipet sample into a slide – choose between the Countess Reusable Slide or the Countess Cell Counting Chamber Slide
Insert the slide into the instrument - Rapid Capture and Auto-Save modes allow cell counting with a single touch. Focus, lighting, and file saving are executed automatically.
Get accurate cell counts
The Countess 3 FL Automated Cell Counter uses trypan blue staining with brightfield illumination combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 × 104 to 1 × 107 cells/mL (the optimal range is 1 × 105 to 4 × 106 cells/mL), which is broader than that of a hemocytometer.
Simple operation with fluorescent application versatility
Operated using an intuitive user interface, automated cell counting with the Countess 3 FL counter gives you the data you need to prepare your sample for the next passage or experiment and allows you to multi-plex with a variety of fluorescent dyes and proteins excited via EVOS light cubes. You no longer need complex, expensive instruments to obtain reliable data.
Transfer data and images to your PC or cloud
Using the USB port on the Countess 3 FL Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. Alternatively, use the Wi-Fi dongle and save results to the cloud for remote access and sharing.













