ResourceLife Sciences
Assessing Therapies for Traumatic Brain or Spinal Cord Injuries
5 Jan 2015A recent study published by Dohi et al. (2014) examined the effects of molecular hydrogen given in drinking water, using a traumatic brain injury (TBI) animal model and conditionally immortalized mouse cerebral pericyte cells (ImBPC). Dohi et al. used a Seahorse Bioscience XF24 Extracellular Flux Analyzer to measure the changes in mitochondrial respiration using the XF Cell Mito Stress Test and the results of the study are summarized here.
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XF Cell Mito Stress Test Kit
Agilent TechnologiesThe XF Cell Mito Stress Test is the gold standard for measuring mitochondrial function.
Seahorse XFe24 Analyzers
Agilent TechnologiesXFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.
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MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.MitochondriaHydrogen