Assessing reader capability for NanoBRET technology

11 Mar 2025

This application note demonstrates the performance of NanoBRET™ technology for detecting protein interactions in living cells, using SpectraMax® Multi-Mode Microplate Readers. By leveraging the high sensitivity and flexible detection options of these instruments, researchers can accurately measure NanoBRET signals and analyze data with SoftMax® Pro Software. The note highlights assay validation across five reader models, showcasing their capability to deliver reliable results with enhanced signal quality and low background.

SoftMax® Pro Microplate Data Acquisition & Analysis Software

Molecular Devices®

SoftMax ® Pro Software is designed to provide the simplicity, flexibility and power required for advanced data analysis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Every step has been optimized for data acquired from a Molecular Devices microplate reader or data imported from another source to simplify analysis and reporting. FDA 21 CFR Part 11 compliance tools are available for regulated laboratories providing end-to-end chain of custody.

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SpectraMax® Microplate Readers

Molecular Devices®

The SpectraMax® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more

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Assessing reader capability for NanoBRET technology