ResourceLife Sciences
Antibody validation
5 Aug 2020Antibodies are generally raised against either proteins (partial or full length), or a short peptide sequence, specific to their target protein. However, how do you know whether they bind to the target against which they were raised? In this application note, Biorbyt outlines the basic validation steps that should be carried out for your antibodies.
Related products
Request Quote for All Products
CD133 antibody
BiorbytRabbit polyclonal antibody to CD133; which is also known as prominin or AC133. CD133 is a marker frequently found on multipotent progenitor cells; including immature hematopoietic stem and progenitor cells. The protein has been extensively used as a stem cell marker for normal and cancerous tissues.
Links
Tags
AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunohistochemistryImmunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples by using antibodies that bind to target antigens. IHC is widely applied in pathology, immunology, hematology and cancer diagnostics. Explore IHC tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Method ValidationMethod validation is the process used to prove that the analytical procedure used for a particular test is capable of providing suitable data.