Antibiotic Screen from Bovine Kidney Juice using Sepra™ C18-E Bulk Sorbent and Gemini® C18 HPLC Columns

16 Feb 2015

Rapid screening tests can be used as part of an efficient program designed to monitor veterinary drug residues in cattle. This application note demonstrates the successful isolation of antibiotics from bovine kidney juice using a non-retentive clean up step followed by analysis by LC/MS/MS with Gemini® C18 HPLC Columns.

Gemini pH Flexible LC Columns

Phenomenex Inc

Setting the Standard for pH Method DevelopmentRugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX™ technology Ideal for analytical and preparative separations of basic and acidic compounds Who needs Phenomenex's Gemini HPLC columns? Method Developers: Extreme pH method flexibility Reproducible performance Purification Chemists: Purify bases at high pH Increased loading Increased retention Increased resolution Lab Managers: Extended column lifetime Consistent results.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibioticsAntibiotics are substances that kill or inhibit the growth of microorganisms, such as bacteria and fungi. They are essential in the treatment of infections and in preventing disease spread. Explore antibiotics research and diagnostic tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ScreeningUsing robotics, data processing and control software, liquid handling devices and sensitive detectors, screening allows a researcher to quickly conduct millions of chemical, genetic or pharmacological tests.
Antibiotic Screen from Bovine Kidney Juice using Sepra™ C18-E Bulk Sorbent and Gemini® C18 HPLC Columns