Gemini pH Flexible LC Columns
Setting the Standard for pH Method DevelopmentRugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX™ technology Ideal for analytical and preparative separations of basic and acid…
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Review Date: 27 Sept 2023 | Phenomenex Inc
Setting the Standard for pH Method Development
Rugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations.
- Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity
- Expect longer column lifetime with patented TWIN-NX™ technology
- Ideal for analytical and preparative separations of basic and acidic compounds
Who needs Phenomenex's Gemini HPLC columns? Method Developers: Extreme pH method flexibility Reproducible performance Purification Chemists: Purify bases at high pH Increased loading Increased retention Increased resolution Lab Managers: Extended column lifetime Consistent results.
Rapid LC-MS/MS method for monitoring bio-relevant levels of per- and polyfluoroalkyl substances (PFAS) in serum
In this application note, a quantitative workflow for the analysis of 22 per- and polyfluoroalkyl substances (PFAS) in serum was developed using a Gemini™ 3µm C18 column coupled with a SCIEX QTRAP® 6500+system. This targeted screening workflow provides a fast analytical method capable of accurately quantifying sub-nanogram per mL levels of PFAS in the human body.
Per- and polyfluoroalkyl substances are pervasive compounds used in a variety of industrial applications and found in a wide range of consumer products such as cookware, stain repellent, flame-retardant, and coatings. PFAS are considered environmental factors due to their persistent and bio accumulating nature. Bioaccumulation of PFAS in the human body resulting from environmental exposure is a growing public health concern. Recent studies have linked PFAS exposure to adverse health outcomes including:
- childhood health complications
- reduced kidney function
- thyroid disease
- hormone suppression
- decreased fertility
- increased cholesterol levels
- diabetes
Reducing PFAS interferences during human plasma and serum analysis with accurate mass spectrometry
In this application note, Phenomenex presents experimental data on the reduction of per- and polyfluorinated (PFAS) interferences in human plasma and serum analysis. PFAS are well known anthropogenic contaminants and are detected worldwide in most environmental media, including air, water and soil. Widespread human exposure has resulted in nearly ubiquitous detection of PFAS in human serum. In order to identify the potential health risks PFAS present to humans, accurate and robust analytical methods are required.
In this analysis, a Gemini™ C18 column coupled with a high resolution, high mass accuracy mass spectrometer was used to mass resolve perfluorooctane sulfonate (PFOS) and perfluorohexane sulfonate (PFHxS) from serum interferences. The EPA 533 PFAS list was selected as the target analytes. The interferences were characterized using their fragmentation patterns and through comparison with MS/MS libraries. Finally, the serum interferences were chromatographically resolved using a 6.5 min gradient.
