Analyze Experimental Results Using Curve Fitting with MetaXpress 6.5 Software

The new Curve Fitting Algorithms in the MetaXpress^® 6.5 (MX 6.5) HighContent Image Acquisition and Analysis Software were designed to facilitate data visualization and the secondary analysis of data. The software allows you to generate a curve of best fit to a series of data points. With multiple functions, including 4-parameter logistic, and multiple curve outputs, such as EC₅₀ and benchmark response, an array of graphs and curves can be generated to best represent data. Here, we show how to generate doseresponse relationship for defining the EC₅₀ values of compounds using a toxicity assay as an example.