ResourceSpectroscopy

Absolute quantification of proteins in snake venom

4 Oct 2021

In this application note, the direct quantification of intact proteins was applied to the analysis of Mozambique spitting cobra (Naja mossambica) venom using capLC-ICP-QQQ and online isotope dilution analysis.

8900 Triple Quadrupole ICP-MS

Agilent Technologies

The Agilent 8900 triple quadrupole ICP-MS (ICP-QQQ) is the world’s most successful and widely used tandem ICP mass spectrometer. Available in a range of configurations to cover applications from routine contract analysis to advanced research and high-purity materials analysis, the Agilent 8900 ICP-QQQ redefines ICP-MS performance, delivering results you can trust.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.ICP-MSInductively Coupled Plasma Mass Spectrometry (ICP-MS) is a powerful analytical technique used to determine the concentrations of elements and their isotopes in a variety of samples, including water, soil, and biological tissues. It involves ionizing elements in the samples with extremely high-temperature Argon (Ar) plasma and then using a mass spectrometer to measure the number of ions based on their mass-to-charge ratio (m/z). Explore ICP-MS systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.
Absolute quantification of proteins in snake venom