SelectScience
Join Free
Accelerating Science
ResourceSeparations

A Sensitive, Specific, Accurate, and Fast LC/MS/MS Method for Measurement of Nicotine, Cotinine, 3-Hydroxycotinine, Nornicotine and Anabasine in Human Urine

Phenomenex Inc
14 Apr 2015

This application note illustrates a method simultaneous analysis of nicotine, two of its major metabolites, cotinine and 3-hydroxycotinine, as well as nornicotine and anabasine from human urine samples. The method described uses Strata™-X-C solid phase extraction (SPE) cartridges for sample clean-up and concentration, followed by fast (<6 min) LC/MS/MS analysis using a Gemini® NX-C18 column.

Related products

Request Quote for All Products

Gemini pH Flexible LC Columns

Phenomenex Inc

Setting the Standard for pH Method DevelopmentRugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX™ technology Ideal for analytical and preparative separations of basic and acidic compounds Who needs Phenomenex's Gemini HPLC columns? Method Developers: Extreme pH method flexibility Reproducible performance Purification Chemists: Purify bases at high pH Increased loading Increased retention Increased resolution Lab Managers: Extended column lifetime Consistent results.

(12)

Links

Phenomenex IncCompany website

Tags

Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.Solid Phase Extraction (SPE)Solid phase extraction (SPE) is a sample preparation technique used to separate and concentrate analytes from complex mixtures. It is widely used in environmental, pharmaceutical, and clinical analysis. SPE provides higher sensitivity and selectivity compared to traditional liquid-liquid extraction methods, making it essential for accurate and reproducible results. Explore SPE tools and systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.UrineThe analysis of urine is important in medicinal, anti-doping, forensic and life sciences applications. The ability to evaluate what someone has ingested through their urine enables fast detection and diagnosis of certain illnesses.

Related resources