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A reliable and simple FIA-MS/MS method for the quantitation of amino acids and acylcarnitines in dried blood spots

Thermo Fisher Scientific
10 Nov 2023

This application note from Thermo Fisher Scientific details a FIA-MS/MS method using the Thermo Scientific™ Vanquish™ MD HPLC and Thermo Scientific™ TSQ Quantis™ MD MS. The method, validated with ClinSpot™ LC-MS/MS Complete Kits, enables the detection of 12 amino acids and 13 acylcarnitines from dried blood spot samples. It fulfills the requirement for semi-quantitative determination of these compounds, crucial for newborn screening.

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Thermo Scientific™ Vanquish™ MD HPLC System

Thermo Fisher Scientific

Develop in vitro diagnostic tests with superior resolution. The Thermo Scientific™ Vanquish™ MD HPLC is the ideal chromatographic separations system for laboratories whose analyte resolution is more critical than sample throughput and batch turnaround time. The small, powerful single channel Vanquish MD HPLC meets analytical needs, as well as space and budget limitations.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.Clinical Mass SpectrometryMass spectrometry is emerging as a superior method of analysis over the longstanding, gold standard of immunoassay. Clinical mass spectrometry systems are increasingly accessible to medical laboratories and are capable of providing results with more speed and less expense than traditional methods. From simple mass spectrometers to IVDR class A compliant, highly sophisticated systems performing liquid chromatography tandem mass spectrometry (LC/MS/MS), find the latest product news, reviews and resources to make a purchasing decision for your laboratory. Clinical StudyProspective biomedical or behavioral research studies on human subjects, following tests in animals, that are designed to answer specific questions about novel vaccines, drugs, treatments, dietary supplements, devices or new ways of using known interventions, generating safety and efficacy data.Dried Blood Spots

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A reliable and simple FIA-MS/MS method for the quantitation of amino acids and acylcarnitines in dried blood spots