ResourceLife Sciences
A high-throughput, multiplex antibody internalization assay
28 Apr 2021Many fluorescence based methods are available to assess internalization; however, performing these assays can prove challenging due to complicated workflows, large sample sizes and the low throughput of analytical methods such as traditional flow cytometry or confocal microscopy.
In this poster, Sartorius describe a high-throughput, multiplex, no-wash assay that measures antibody internalization, specificity, and cell health from a single 30 uL assay volume in 96- and 384- well formats, with the iQue® platform.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.