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A High-Sensitivity UPLC/MS/MS Method for the Quantification of Desmopressin in Rat Plasma
A High-Sensitivity UPLC/MS/MS Method for the Quantification of Desmopressin in Rat Plasma
19 Aug 2015This application note describes a highly specific and sensitive methodology for the quantification of desmopressin in plasma using solid phase extraction (SPE) followed by reversed-phase UPLC® coupled to tandem quadrupole mass spectrometry. Desmopressin is a peptide containing nine amino acids, a form of the normal human hormone arginine vasopressin that reduces urine production, and is most commonly prescribed for the treatment of diabetes insipidus or nocturnal enuresis. The low circulating levels of desmopressin requires a high-sensitivity assay to accurately define its pharmacokinetics in humans, especially pediatrics. The combination of solid-phase extraction methodology, UPLC chromatography, and Xevo TQ-S mass spectrometer has facilitated the development of an assay for desmopressin with a LLOQ of 1 pg/mL in plasma.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Animal ModelsThe use of non-human animals in experiments or behavorial observations. The research is conducted inside universities, medical schools, pharmaceutical companies, farms, defence establishments, and commercial facilities that provide animal-testing services to industry. It includes pure research such as genetics, developmental biology, behavioral studies, as well as applied research such as pharmaceutical testing in pre-clinical, before human, studies. DiabetesDiabetes is a metabolic disorder characterized by high blood sugar levels, either due to insufficient insulin production or resistance to its effects. Ongoing diabetes innovation focuses on developing better diagnostic tools, treatments, and preventive measures. Browse our peer-reviewed product directory to find the best diabetes diagnostic tools, compare products, check reviews, and get pricing directly from manufacturers.Amino AcidsSolid Phase Extraction (SPE)Solid phase extraction (SPE) is a sample preparation technique used to separate and concentrate analytes from complex mixtures. It is widely used in environmental, pharmaceutical, and clinical analysis. SPE provides higher sensitivity and selectivity compared to traditional liquid-liquid extraction methods, making it essential for accurate and reproducible results. Explore SPE tools and systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.PlasmaUrineThe analysis of urine is important in medicinal, anti-doping, forensic and life sciences applications. The ability to evaluate what someone has ingested through their urine enables fast detection and diagnosis of certain illnesses.
