Western Blotting > Western Blotting Labeling and Detecting Reagents Products & Reviews

The Quick Western Kit provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step.

IRDye 680RD is the near-infrared fluorescent dye of choice for applications in the 700 nm wavelength, especially Western blots, cell-based assays such as the In-Cell Western assay, and small animal imaging experiments.

IRDye Infrared Dyes and near-infrared (NIR) fluorescent imaging deliver enhanced sensitivity due to low background autofluorescence in the near-infrared region, therefore, giving a higher signal to noise ratios.

CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.

Visualize and quantify the total protein content on Western blot membranes with Revert 700 Total Protein Stain. Revert 700 Total Protein Stain provides linear, proportional signal across a broad range of sample concentrations.

IRDye Labeling Kits come in 3 forms and allow you to label your own peptides, antibodies, or proteins.

The Odyssey Pen is suitable for writing on membranes to be scanned on any of the Odyssey Imagers.

CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.

Imaging probes conjugated to IRDye 800CW and IRDye 700DX have propelled more than 20 Phase I and Phase II clinical trials worldwide.

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes.

The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence of HRP. The ECL Western Blotting Substrate is a highly sensitive non-radioactive,…

A high-throughput fluorescence-based assay for protein stability screening with higher tolerance for detergents and other protein additives compared to commonly used Thermal Shift dyes.

The new family of Luminata Western HRP substrates are a family of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. Pour directly onto the Western membrane without worrying about pipetting error. The Luminata™ Classico, Crescendo and Forte substrates cover a broad range of sensitivities.Premixed for convenience and to avoid cross contamination of the reagents.Broad range of sensitivities…

Anti-cdc42 is a mouse monoclonal IgG1 that recognizes cdc42 from human, mouse, rat and dog

This kit provides the reagents required for the detection of citrulline-containing proteins by Western blot analysis with the anti-Citrulline antibody. Detection is a two-step process in which citrulline-containing proteins immobilized on nitrocellulose or PVDF are formaldehyde-cross linked to the membrane to improve protein retention, followed by modification of citrulline residues with 2,3-butanedione monoxime and antipyrine…

The OxyBlot™ Protein Oxidation Detection Kit provides the chemical and immunological reagents necessary to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.

Contains 100 ml Clarity Western Peroxide Reagent and 100 ml Clarity Western Luminol/Enhancer reagent

A new member of the SERVA Light chemiluminescence substrate kits family

Western blotting is a widely used technique to detect levels of protein expression in cell or tissue extract. This technique measures protein levels in a biological sample through antibody binding to a specific protein of interest.   Features: Detects Small Amounts of Protein – delivers a sensitive and robust signal for standard protein detection Convenient, Cost-effective 500 ml Sizing – means you can p…

EZ-ECL Enhanced Chemiluminescence Detection Kit for HRP

BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid

2 ml, HRP-Avidin conjugate for detection of biotinylated SDS-PAGE standards

1 ml, blotting-grade alkaline phosphatase (AP) avidin conjugate

0.5 ml, streptavidin alkaline phosphatase conjugate

The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the assay.