Enzymes > Reverse Transcriptase Products & Reviews

100 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water

HighTherm™ Reverse Transcriptase

Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

The Agilent AffinityScript Multiple Temperature Reverse Transcriptase (RT) system is optimized to produce high cDNA yields over the broadest temperature range. During analysis the multi-temp RT device also ensures high affinity to primer-template complexes. cDNA can be synthesized in amounts up to 20 kb.

Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The Just cDNA Double-Stranded cDNA Synthesis Kit generates high-quality, blunt-ended cDNA, perfect for use in non-directional cDNA libraries.  Makes blunt double-stranded cDNA  Can be used for in vitro transcription  Perfect for nondirectional cDNA libraries

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibit…

The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR. The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC). Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.

1 ml, one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 100 x 20 µl reactions

100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

25 x 20 µl reactions, includes 100 µl iScript Advanced 5x Reaction Mix, 25 µl iScript Advanced Reverse Transcriptase

25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 200 µl No-RT control supermix; 12.5 µl DNase; 150 µl DNase buffer

100 x 20 µl reactions, 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 400 µl No-RT control supermix; 50 µl DNase; 150 µl DNase buffer

25 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water

50 x 50 µl reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water

25 x 20 µl reactions, 100 µl 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

100 x 20 µl reactions, 4 x 100 µl 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

25 x 20 µl reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

100 x 20 µl reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

250 x 50 µl reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water

Appropriate for COVID-19 detection.

EpiScript™ Rnase H- Reverse Transcriptase (EpiScript RT) is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.

25 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 25 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water