Next Generation Sequencing > NGS Preparation Kits Products & Reviews

Accurate and sensitive quantification of DNA, RNA, and protein with flexible throughput

Agilent’s market leading SureSelect platform provides a complete portfolio of catalog and custom products, providing the flexibility you need from discovery to follow-up studies. Agilent’s custom solutions allow you to target your regions of interest using our free web portal SureDesign. Custom solutions are available for DNA and RNA target enrichment.

Agilent SureSelect DNA panels are predefined designs which focus on targeted gene sets for specific applications. Agilent offers two human panels, the SureSelect Human Kinome panel and SureSelect X-Chromosome panel. The SureSelect Human DNA Kinome panel targets a comprehensive set of kinases and kinase related genes for enrichment, targeting over 500 kinases and 612 genes. The SureSelect X-Chromosome panel targets all of the g…

Agilent's SureSelect market leading platform provides a complete portfolio of catalog and custom products, providing the flexibility you need, for your discovery to follow-up studies. Agilent™s SureSelect Target Enrichment System is a highly efficient hybrid selection technique for optimizing next-generation sequencing. It is available for the Illumina GA and HiSeq, Applied Biosystems SOLiD, and 454 Life Sciences FLX and GS J…

Agilent SureSelect All Exon kits are the most widely used target enrichment solution for exome sequencing. SureSelect Human All Exon V5 (targeting coding regions only) and V5+UTRs (targeting coding regions plus UTRs) have been updated to provide the greatest sequencing efficiency and enable samples ready for sequencing the next day. Greater coverage with less sequencing for lower overall costs – up to 60% less sequencing than…

Now delivering unparalleled productivity. Enrich any gene panel with our simple, fast and high-performing protocol! Only HaloPlex enables generation of paired-end reads, even when sequencing single-end, with the Paired-End by Design System. Overcome the limitations of Multiplex PCR >20,000 amplicons per tube, with inclusion of multiple amplicon coverage of each base No primer cross-reactivity or dropouts Improved artifact filt…

Agilent provides the most comprehensive menu of FISH probes for rapid identification of a wide range of chromosomal aberrations across the genome. Our catalog probes target relevant regions for a wide range of applications such as detection of aneuploidy, constitutional microdeletion syndromes, various rearrangements in oncology, and constitutional cytogenetics. Agilent's Comprehensive Menu of FISH Probes Provides: Coverage o…

The HaloPlex Cancer Research Panel enables fast, simple, and efficient analysis of genomic regions of interest for cancer research for a large number of sample types, including FFPE. HaloPlex Cancer is uniquely suited to high performance with cancer research samples, which are commonly preserved as formalin fixed and paraffin embedded (FFPE). This FFPE process often results in highly fragmented DNA, resulting in insufficient…

HaloPlex technology provides outstanding performance, streamlined workflow, and low sample input requirements for next generation sequencing of human exomes. The HaloPlex Exome has been optimized to provide comprehensive coverage of the coding regions of the human genome with minimal hands on time and requires only 200ng of input DNA. The Haloplex Exome performance and workflow is ideal for clinical research: • Outstanding cov…

Agilent Haloplex custom kits offer the flexibility to create custom panels for any genomic regions of interest. A unique and efficient library prep-free workflow enables complete target enrichment in less than 6 hours from just 200 ng of input DNA.

Agilent HaloPlex panels are predefined designs which focus on targeted gene sets for specific applications. Agilent offers two catalog HaloPlex panels, the Cancer Research Panel and the Cardiomyopathy panel.The HaloPlex Cardiomyopathy (catalog) is a next generation sequencing target enrichment panel designed specifically for inherited forms of cardiomyopathy. Following a careful review of cardiomyopathy publications as well as…

The SureSelect Focused Exome is a highly targeted design that enables analysis of only disease-associated targets, enabling superior coverage of disease-associated regions even on benchtop sequencers. This design provides 20 or more reads for 95% of targets at 1.5Gb (100x) of sequencing and 98% of targets with 3Gb (200x) of sequencing.

Designed for smaller studies, the SureSelect DNA Capture Arrays complement Agilent's in-solution SureSelect Target Enrichment System, which is designed for medium to large-scale NGS studies of tens through thousands of samples, including automated high-throughput workflows.

Efficient, and uniform hybridization-based capture for Whole Exome Sequencing Combining more than a decade of probe-design experience with an improved probe-manufacturing process, the new KAPA HyperExome probe pools enable efficient, uniform hybridization-based capture for Whole Exome Sequencing (WES).

KAPA HyperPETE (Primer Extension Target Enrichment) is a novel hybridization capture technology designed to employ primer extension reactions to specifically capture and release target library molecules for sequencing.

Amplify a CRISPR guide sequence derived from a CROP-style vector alongside your standard RNA Sequencing Library.

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high-fidelity polymerases and polymerase blends.

The AAVX and AAV9 Total Capsid Quantification Kits extend the Amperia™ Protein Quantification System for streamlined measurement of adeno-associated virus (AAV) vectors. Incorporating CaptureSelect™ affinity reagents from Thermo Fisher Scientific, the kits provide robust, reproducible assays for both broad serotype coverage and AAV9-specific quantification.

The Protein A and Protein G Quantification Kits extend the Amperia™ Protein Quantification System for streamlined measurement of antibodies across subclasses and species. Using Fc capture with an inverse-occupancy format, the kits deliver reproducible titres directly from crude or clarified samples, with up to 320 tests per kit.

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

SPRIselect is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection for fragment library preparation for Next Generation sequencing.

New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction.NEBNext® dsDNA Fragmentase Reaction Buffer Components: 20mM Tris…

NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl…