Protein Purification > Affinity Purification Products & Reviews

PhyTip® columns use dual-flow chromatography within pipette tips to automate purification workflows. This technology delivers high reproducibility and exceptional sample purity for efficient, reliable results.

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin.

StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.

The InterPlay Mammalian TAP System allows you to recover interacting proteins from mammalian cells. This method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP). 

The InterPlay Mammalian TAP System allows you to recover interacting proteins from mammalian cells. This method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP). 

The InterPlay Mammalian TAP System allows recovery of interacting proteins from mammalian cells. The method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP).

The InterPlay Mammalian TAP System allows recovery of interacting proteins from mammalian cells. The method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP).

High-quality, pure RNA Eliminates tedious, time-consuming lab procedures. Multiple functional tests. Most Economical source of RNA  High-quality, pure RNA  Eliminates tedious, time-consuming lab procedures  Multiple functional tests  Most Economical source of RNA

The Affinity protein expression and purification system provides a simple, gentle, and effective method for high-level protein expression and purification. A complete Affinity protein expression and purification system includes calmodulin (calcium-modulated protein or CaM) affinity resin and pCAL expression vectors, containing a calmodulin-binding peptide (CBP) tag.

The Affinity Removal LC Column - Human Albumin is designed to chromatographically remove the most interfering high-abundance protein (albumin) from human samples (e.g., plasma, serum, urine, or cerebrospinal fluid). They are compatible with most FPLC or HPLC pumps or instruments, including Agilent 1200/1100 instruments.

It can be challenging to purify sufficient high-quality protein from mammalian cells. Boost protein recovery 30-60% with an optimized covalent protein tag, called HaloTag®. This complete kit offers strong improvements in recovery, purity, cost and protein functionality over historical FLAG® and poly-His tag purification strategies.

The MagneHis™ Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins.   Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format,…

HaloLink - Promega Magnetic Beads for Covalent immobilization of HaloTag® fusion proteins.   Features of the Promega HaloLink Magnetic Beads: • Magnetic-Based: Easy and quick immobilization of HaloTag® fusion proteins with minimal loss of sample material. • Covalent Bond: Allows stringent washing of immobilized HaloTag® fusion protein, removing nonspecific proteins and enhancing overall data.  • Multiple Appli…

A comprehensive portfolio of high-quality resins, columns, buffers, and accessories essential for the purification and analysis of biomolecules across discovery and process development scales.

An affinity chromatography resin for purifying recombinant proteins using the self-cleaving Cytiva Protein Select tag.

Streptavidin isolated from Streptomyces avidinii covalently bound to agarose.

QuickPick™ GST kit for proteins, 48 preps (62411)The ready-to-use QuickPick GST glutathione affinity kit is intended for the purification of GST (glutathione-S-transferase)-tagged proteins. It is optimized for use with the PickPen 1-M magnetic tool, and at least 40 ug tagged protein can be purified per prep.

Introducing an all-in-one centrifugal device for the protein purification workflow.Traditional protein purification is a long process with many steps and multiple devices, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. No matter what your workfl…

cOmplete His-Tag Purification Column - Prepacked columns with EDTA-compatible resin for the purification of poly-histidine-tagged proteins. cOmplete His-Tag Purification Columns are available in 2 different sizes and prepacked with cOmplete His-Tag Purification Resin. cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC matrix (Immobilized Metal Affinity Chromatography) for convenient single-step purificati…

Magnetic Bead-Based Isolation of Proteins Millipore’s new PureProteome magnetic beads ensure the rapid and reproducible isolation of proteins. Unlike conventional methods that require centrifugation to pellet the beads then careful aspiration to avoid sample loss, PureProteome magnetic beads are isolated using a magnetic rack. This allows for the total removal of buffers for complete recovery of beads no sample dilution. You…

Purification Consumables and Custom Products.We understand that your purification challenges are unique. That’s why we offer you a variety of options to help you solve them. Whether you seek the up-front cost savings and versatility of packing your own columns, the consistent quality and time savings of pre-packed columns, or the ease associated with having us purify material for you; Sigma-Aldrich is your purification partner…

FG beads are magnetic nanoparticles, suitable for various applications. There are many kinds, which have different functional groups and pre-immobilized proteins.

FG beads® are beads approximately 0.2 μm in diameter that are composed of multiple ferrite particles coated with a special polymer called poly-GMA (glycidyl methacrylate). The FG beads® that are manufactured using this original technology are used as matrices for affinity purification and provide characteristics that are superior to conventional matrices.

FG beads® are beads approximately 0.2 μm in diameter that are composed of multiple ferrite particles coated with a special polymer called poly-GMA (glycidyl methacrylate). The FG beads® that are manufactured using this original technology are used as matrices for affinity purification and provide characteristics that are superior to conventional matrices.

FG beads® are beads approximately 0.2 μm in diameter that are composed of multiple ferrite particles coated with a special polymer called poly-GMA (glycidyl methacrylate). The FG beads® that are manufactured using this original technology are used as matrices for affinity purification and provide characteristics that are superior to conventional matrices.