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Wheat Germ Agglutinin (WGA) Conjugates

Biotium

A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes, biotin, or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells.

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CF® Dye Tyramide

Biotium

Fluorescent CF® Dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

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Heat&Run gDNA Removal Kit

ArcticZymes

The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR. The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC). Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.

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Endonucleases DNA-specific

ArcticZymes

Double-Strand Specific dsDNase from ArcticZymes is ideal for removing contaminating DNA from PCR master mixes, preventing false positives in bacterial typing. Recombinantly produced in Pichia pastoris, it cleaves dsDNA with 5000-fold higher specificity than ssDNA. Easily inactivated by moderate heat (65°C), it allows for direct addition of DNA templates post-cleanup, ensuring accurate PCR results.

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AZtaq™ DNA Polymerase

ArcticZymes

AZtaq™ DNA Polymerase, derived from Thermus aquaticus, is a high-quality, thermostable enzyme ideal for PCR applications. It catalyzes the synthesis of complementary DNA using a primed template and has 5’-3’ exonuclease activity but lacks 3’-5’ proofreading. AZtaq is compatible with dUTP, allowing efficient carry-over contamination removal when used with Cod UNG.

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Endonucleases Non-Specific, HL-SAN

ArcticZymes

HL-SAN efficiently removes nucleic acids from buffers in protein purification, excelling in high-salt conditions to reduce aggregation. Ideal for removing nucleic acids from proteins with high DNA/RNA affinity, it performs well during lysis, early purification stages, and high-salt eluates. This cold-adapted enzyme works effectively at ambient temperatures and during overnight digestion at 4°C. Triton free available.

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