DAB (3,3-diaminobenzidine tetrahydrochloride), is used as a chromogen substrate in immunoblotting.DAB produces a brown colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 10 mg of DAB and dissolves in distilled water in less than 5 minutes. Stability life time is 18 months at 4°C.
Fast Red is used as a chromogen substrate in immunoblotting and immunohistochemistry using alkaline phosphatase antibody conjugates. Each tablet contains 0.5 mg naphthol phosphate, 2 mg FAST RED chromogen and 0.4 mg levamisole, an inhibitor of endogenous enzyme activity. Stability life time is 18 months at 4°C.
The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add equal amounts of 1 .0 M s…
The liquid BCIP/NBT is the most commonly used substrate for colorimetric determination of Alkaline Phosphatase (AP) activity in membrane tests. We offer an aqueous buffered solution containing NBT (NitroBlue Tetrazolium) and BCIP (Bromo-Chloro-Indolyl Phosphate) forming a blue/purple precipitate at the positive sites. The colour develops when AP catalyses the dephosphorylation of BCIP. The intense blue/purple precipitate is…
Alpha is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable detection of unique biological events, offering maximum flexibility.
This kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of Total ADP in 10µl of rat serum, plasma or cell culture supernatants and does not cross react with Rat ADP. Format:ELISA / EIA;Sample Type:Serum, Plasma, Cell Culture;Sample Size:10 µl;Size:96 Wells; Range:0.5 - 32 ng/ml; Sensitivity:50 pg/ml; Incubation Time:3 hours 20 minutes
Mannan-binding lectin (MBL) is a plasma protein that activates the complement system on binding to invading pathogens It also has more subtle immunomodulatory effects. Genetically determined MBL deficiency has been shown to be associated with increased susceptibility to infections in conditions such as: Immunosuppression, during cancer chemotherapy, after organ transplantation, immature adaptive immune system and in early chi…
Assess serum Gc-globulin levels to obtain important information about prognosis and progress in critically ill patients suffering from fulminant liver failure, Acetaminophen overdose, multiple trauma and Sepsis. Using our Gc-globulin (Actin-free) ELISA Kit you can determine Gc-globulin levels in serum or plasma in less than 1 hour. The fast procedure and easy-to-use ELISA format makes this kit ideal for prognostic assessment a…
NGAL is a new early marker for acute renal injury. With the new NGAL ELISA KIT fro BioPorto Diagnostics, urine, plasma or serum samples can be analysed in a standard laboratory with conventional ELISA equipment. All components are stored at 4ºC and all incubations are performed at room temperature. The kit contains ready-to-use calibrators, reagents and pre-coated well strips, which greatly reduces the hands-on time. The sim…
NGAL (also known as neutrophil gelatinase-associated lipocalin or lipocalin-2) is a new early marker for acute renal injury. Increased levels of NGAL from acute renal injury can be detected in both urine and blood within two hours of the insult. With the new NGAL ELISA KIT, urine, plasma or serum samples can be analysed in less than 1 hour in a standard laboratory with conventional ELISA equipment. All components are stored at…
Rat NGAL - a marker for nephrotoxicity in pre-clinical trials. With the new Rat NGAL ELISA KIT, urine, plasma or serum samples can be analysed in a standard laboratory with conventional ELISA equipment. All components are stored at 4ºC and all incubations are performed at room temperature. The kit contains ready-to-use calibrators, reagents and pre-coated well strips, which greatly reduces the hands-on time. The simple test pr…
