PCR and Thermal Cycling > PCR Reagents Products & Reviews

Optimized primers for different human miRNA that were selected based upon their roles in cancer and development.  Detects mature miRNA  Detects human, mouse, rat, worm and other species  Highly-specific sequences assure single nucleotide discrimination

Optimized primers for different human miRNA that were selected based upon their roles in cancer and development.  Detects mature miRNA  Detects human, mouse, rat, worm and other species  Highly-specific sequences assure single nucleotide discrimination

Optimized primers for different human miRNA that were selected based upon their roles in cancer and development.  Detects mature miRNA  Detects human, mouse, rat, worm and other species  Highly-specific sequences assure single nucleotide discrimination

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

The Prime-It Fluor fluorescence labeling kit generates directly fluoresceinated DNA probes using random 9-mer primers and Exo-Klenow to incorporate fluor-12-dUTP into the probe fragments.  Generates high-specific-activity probes in 2 minutes  Fluorescently labeled probes also available  Produces labeled fragments 400 bp to 10 kb nucleotides in length  Performs equally well with [32P]dATP and [32P]dCTP  Performs well w…

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

These reactions buffers contain the optimal magnesium concentration for efficient PCR.  Ideal for optimal PCR performance  Standard 10x formulation  Optimized for use with specific PCR enzymes

The passive reference dye can be added to fluorescence-based quantitative PCR (qPCR) assays to compensate for non-PCR related variations in fluorescence. Product comes in 10 vials of 100 µL. 1mM for controlling the final dye concentration.

Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates. It includes an enzyme blend of Platinum Taq DNA Polymerase and a proofreading enzyme, plus AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime Taq DNA Polymerase, High Fidelity, you'll get: • The…

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution.   The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA. The…

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.   Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits:   Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…

Sensitive Quantitation of Single-Stranded DNARequires less DNA than spectrophotometry

cGMP-Grade Solution of dNTPs Pretested in PCR- Two concentrations available: 10mM and 25mM

Optimized PCR master mix: Just add template, primers and water

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

Modifications that enhance the performance of oligonucleotides

A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.

An optimized photoactivation device designed for photolysis of PMAxx™- and PMA- treated samples in viability PCR.

PCR Water is a molecular biology grade product suitable for sample dilution, and use as an ultra-pure diluent for PCRs and other molecular biology reactions.

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

A high-performance dye-based qPCR master mix containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. With low or high ROX.

A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.

A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.