Gene Expression and Molecular Cloning Products & Reviews

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.  Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

The AdEasy Viral Titer Kit is a simple enzyme-linked immunoassay for the determination of adenoviral titers. The immunoassay detects an adenoviral capsid protein called hexon, which is required for adenoviral replication. The AdEasy viral titer kit can determine the titer of your stocks in 24 to 48 hours, compared to two weeks for traditional endpoint dilution assays.

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

AffinityScript Multiple Temperature Reverse Transcriptase (RT) produces high cDNA yields over the broadest temperature range with high affinity to primer/template complexes. It delivers high cDNA yield when amplifying low RNA input amounts and resolving RNA secondary structure using temperatures up to 55°C.

Alien Reference RNA QRT-PCR Detection Kit for Monitoring the Overall Performance of QRT-PCR Assays.

Alien Reference RNA QRT-PCR Detection Kit for Monitoring the Overall Performance of QRT-PCR Assays.

The Big Blue transgenic rodent mutagenesis assay is an in vivo system for assessing the genetic toxicity of test compounds.  Reliable and widely used in vivo mutagenesis assay  LacZ and cII gene incorporated into Chromosome 4

The Big Blue transgenic rodent mutagenesis assay is an in vivo system for assessing the genetic toxicity of test compounds.  Reliable and widely used in vivo mutagenesis assay  LacZ and cII gene incorporated into Chromosome 4

The Big Blue transgenic rodent mutagenesis assay is an in vivo system for assessing the genetic toxicity of test compounds.  Reliable and widely used in vivo mutagenesis assay  LacZ and cII gene incorporated into Chromosome 4

The Big Blue transgenic rodent mutagenesis assay is an in vivo system for assessing the genetic toxicity of test compounds.  Reliable and widely used in vivo mutagenesis assay  LacZ and cII gene incorporated into Chromosome 4

The Big Blue transgenic rodent mutagenesis assay is an in vivo system for assessing the genetic toxicity of test compounds.  Reliable and widely used in vivo mutagenesis assay  LacZ and cII gene incorporated into Chromosome 4

The Big Blue transgenic rodent mutagenesis assay is an in vivo system for assessing the genetic toxicity of test compounds.  Reliable and widely used in vivo mutagenesis assay  LacZ and cII gene incorporated into Chromosome 4

The Big Blue transgenic rodent mutagenesis assay is an in vivo system for assessing the genetic toxicity of test compounds.  Reliable and widely used in vivo mutagenesis assay  LacZ and cII gene incorporated into Chromosome 4

The Big Blue transgenic rodent mutagenesis assay is an in vivo system for assessing the genetic toxicity of test compounds.  Reliable and widely used in vivo mutagenesis assay  LacZ and cII gene incorporated into Chromosome 4

The Big Blue transgenic rodent mutagenesis assay is an in vivo system for assessing the genetic toxicity of test compounds.  Reliable and widely used in vivo mutagenesis assay  LacZ and cII gene incorporated into Chromosome 4

Easy-A high-fidelity cloning enzymes combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.  

The Agilent High Sensitivity Protein 250 kit provides protein detection in the low picogram range. The kit is ideally suited to solve typical protein QA/QC questions and to comply with ICH and FDA guidelines.

The Agilent High Sensitivity Protein 250 kit provides protein detection in the low picogram range. The kit is ideally suited to solve typical protein QA/QC questions and to comply with ICH and FDA guidelines.

Agilent’s High-Specificity miRNA QRT-PCR Detection Kit is a high-specificity reagent system for the detection of miRNA that delivers sensitive and reproducible results with significantly higher specificity than most of our direct competitors. 

Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems.  When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element  

Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems.  When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element.