Gene Expression and Molecular Cloning Products & Reviews

Our CRISPR Design Tool enables an intuitive one-stop location for guide RNA design and ordering. Utilize the design tool to order guide RNAs for targeted gene knockout or location-specific HDR-mediated genome editing.

Optimize your CRISPR-Cas9 experiments with our specific controls and primers

A direct and efficient set of tools to study a gene’s function by overexpression in its native context

Baculovirus cassette vector pAc-kappa-CH3 for the expression of human, humanized or chimeric IgG (kappa) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fv antibody gene fragments.

The safe alternative to using live coronavirus

The safe alternative to using live coronavirus

Direct translocation of proteins into living cells

Pre made lentiviral particles for flourescent proteins

Guaranteed editing with predesigned gRNA designs

Use our proprietary algorithms to design custom Cas9 guide RNAs

Guide RNAs (gRNAs) contain the target-specific sequence for guiding Cas9 protein to a genomic location.

Guide RNAs (gRNAs) contain the target-specific sequence for guiding Cas9 protein to a genomic location.

Guide RNAs (gRNAs) contain the target-specific sequence for guiding Cas9 protein to a genomic location.

Hybridizes to crRNA to activate the Cas9 enzyme.

Hybridizes to crRNA to activate the Cas9 enzyme.

urified carrier DNA that improves delivery of Cas9 ribonucleoprotein (RNP) by electroporation.

Purified carrier DNA that is required for efficient delivery of Cpf1 ribonucleoprotein (RNP) by electroporation.

For detection of on-target or known off-target CRISPR events in cultured cells

An advanced data analysis pipeline for quantifying your genome editing results

A small molecule compound that has demonstrated an ability to increase the rate of homology-directed repair (HDR).

Deep, targeted amplicon sequencing with highly multiplexed panels for sequencing on Illumina platforms.

Complete synthetic genes with 100% sequence verification are provided in a plasmid cloning vector and ready to use in a variety of applications.

Double-stranded DNA fragments for quick and cost-effective cloning

Sigma-Aldrich ® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including microinjection, electroporation, and lipofection.

With the largest portfolio of Cas9 proteins available, Merck make it easy to find the perfect nuclease for your gene editing experiment.