Thermostable DyNAzyme™ I DNA Polymerase is isolated and purified from Thermus brockianus, which is a Finnzymes proprietary strain. DyNAzyme I DNA Polymerase is more thermostable and tolerates DMSO better than Taq based polymerases.DyNAzyme I DNA Polymerase has a half-life of 3 hours at 96 °C. DyNAzyme I DNA Polymerase contains 5’-->3’ exconuclease activity but lacks 3'-->5' proofreading activity. DyNAzyme I DNA Polymerase is p…
Thermostable DyNAzyme™ II DNA Polymerase is isolated and purified from E.coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus which is a Finnzymes proprietary strain. DyNAzyme II DNA Polymerase is more thermostable and tolerates DMSO better than Taq based polymerases. DyNAzyme II DNA Polymerase has a half-life of 2.5 hours at 96 °C. DNA Polymerase contains 5’-->3’ exconuclease ac…
DyNAzyme™ EXT DNA Polymerase is an optimized mixture of DyNAzyme™ II DNA Polymerase and a proofreading enzyme. It is a versatile and easy-to-use enzyme especially suited for handling demanding reaction conditions without time-consuming optimization. DyNAzyme EXT Polymerease offers exceptional performance that Taq based mixtures cannot match.
Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I. Highlights • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers A…
Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity. Highlights • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment (see Reference1) • Active in Therm…
Thermo Scientific T4 Polynucleotide Kinase (PNK) is a tetrameric protein composed of four identical subunits. It is widely used in molecular biology to radiolabel nucleic acids and to add a phosphate to the 5'-end of dephosphorylated nucleic acids to facilitate ligations. Features • Phosphorylates 5’ ends • Transfers 32P to DNA and RNA
Thermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.
Thermo Scientific Shrimp Nuclease is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. The enzyme has a high preference for double-stranded DNA, and can be used to selectively degrade dsDNA, leaving ssDNA and RNA intact. The enzyme is inactivated after 15 minutes at 65 °C.
Thermo Scientific RNase A (ribonuclease A) is supplied as a chromatographically pure freeze-dried powder, salt-free for easy reconstitution in most buffers.
Thermo Scientific Ribonuclease H (RNase H) is an endonuclease that degrades the RNA portion of DNA-RNA hybrids. It hydrolyses phosphodiester bonds of RNA in RNA:DNA heteroduplexes. It produces products with 3’-OH and 5’-phosphate. RNase H will not degrade single or double-stranded DNA or RNA.
Thermo Scientific Shrimp Alkaline Phosphatase (SAP) catalyzes the dephosphorylation of 5´ phosphates from DNA and RNA and is completely and irreversibly inactivated by heating at 65°C for 15 minutes. SAP is active on 5´ overhangs, 5´ recessed and blunt ends.
A cell-permeable (thienothienyl)amino-acetamide compound that displays anti-inflammatory properties. Acts as a potent, reversible, ATP-competitive, and highly selective inhibitor of IKK-2 (IC50 ~3-12 µM for IKK-2 homodimer, IKK-1/IKK-2 heterodimer, and IKK-2). Its specificity has been confirmed using a panel of 31 other kinases, including IKK isoforms IKK-1, IKK-i, and TBK-1 (IC50 > 200 µM). Shown to specifically block NF-kB-d…
Finnzymes’ novel PowerCut™ Dicer produces a pool of 25–27 nucleotide-long siRNA molecules from any dsRNA. This efficient endoribonuclease originating from Giardia intestinalis outperforms all other siRNA producing enzymes by cleaving the substrate with 100 % efficiency yet leaving the resulting siRNA intact. The high quality siRNA produced is ideal for gene silencing studies, for example. Pooled siRNAs are also preferred over…
Top DNA polymerase is a novel thermostable DNA polymerase that is more processive than Taq DNA polymerase. In fact, the extension rate of Top DNA Polymerase is > 3 x that of Taq DNA Polymerase! Top DNA Polymerase can be used for a variety of PCR applications (including TA cloning) and is a robust enzyme for everyday PCR. It contains no proofreading or 5’-3’ Exonuclease activity.Features and Benefits• Fast: More than three tim…
Get more cDNA in less time with CycleScript Reverse Transcriptase. CycleScript is a versatile reverse transcriptase - applicable to both conventional Reverse Transcription and Cyclic Reverse Transcription (Cyclic RT, patent pending – for cDNA amplification). It features high activity across a wide range of temperatures from 37 to 55°C; therefore, reverse transcription is carried out like PCR. The Cyclic RT reaction is composed…
Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme is able to use an RNA molecule as a template and synthesize a double-strand DNA. M-MLV Reverse Transcriptase is isolated from a E.coli strain containing a recombinant clone. It is ideal for use of first-strand synthesis cDNA from RNA molecules and for cDNA synthesis for Reverse Transcriptase PCR and qRT-PCR.
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will ligate DNA from blunt-end and cohesive-end termini (Including sticky ends for TA Cloning) as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids. T4 DNA Ligase is isolated from a recombinant E.coli strain.
