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Recombinant, high-purity endonucleases for genome editing experiments

Expand your options for genome editing with the Alt-R CRISPR-Cas12a System.

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Detect microbial deoxyribonuclease enzymes, particularly from staphylococci, with Thermo Scientific™ Oxoid™ DNASE Agar (Dehydrated).

Recombinant PURedit Cas9 protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments

Recombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

Recombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Thermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.

Thermo Scientific rDNase I is produced through a process that contains no animal by-products. The enzyme is provided free from any detectable RNase activity or animal derivatives. rDNase I has a molecular weight of approximately 30kDa. As an endodeoxyribonuclease, DNase I hydrolyses phosphodiester linkages yielding di-, tri-, and oligonucleotides with a 5' phosphate and a free 3' hydroxyl. DNase I has been shown to act on sing…

Thermo Scientific Shrimp Nuclease is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. The enzyme has a high preference for double-stranded DNA, and can be used to selectively degrade dsDNA, leaving ssDNA and RNA intact. The enzyme is inactivated after 15 minutes at 65 °C.

Thermo Scientific RNase A (ribonuclease A) is supplied as a chromatographically pure freeze-dried powder, salt-free for easy reconstitution in most buffers.

Thermo Scientific Ribonuclease H (RNase H) is an endonuclease that degrades the RNA portion of DNA-RNA hybrids. It hydrolyses phosphodiester bonds of RNA in RNA:DNA heteroduplexes. It produces products with 3’-OH and 5’-phosphate. RNase H will not degrade single or double-stranded DNA or RNA.

Properties: Endoribonuclease that preferentially cleaves 3' to G. Guanosine-3'-phosphates and oligonucleotides with guanosine-3'-phosphate terminal groups are the final products. Substrate is single-stranded RNA. Source: Aspergillus oryzae Volume activity: approx. 105 U/ml Unit definition: One unit according to Egami releases a sufficient quantity of acid-soluble oligonucleotides to produce an absorbance increase of 1.0 at 260…

Finnzymes’ novel PowerCut™ Dicer produces a pool of 25–27 nucleotide-long siRNA molecules from any dsRNA. This efficient endoribonuclease originating from Giardia intestinalis outperforms all other siRNA producing enzymes by cleaving the substrate with 100 % efficiency yet leaving the resulting siRNA intact. The high quality siRNA produced is ideal for gene silencing studies, for example. Pooled siRNAs are also preferred over…