The SIRTainty™ Class III HDAC Assay utilizes a novel patent pending technology for the sensitive detection of all known sirtuin family members. This easy to perform assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC enzymes. Unlike conventional assays that are dependent upon a s…
ICT’s Green Cathepsin B Assay allows researchers to detect and monitor intracellular cathepsin activity over time in vitro using live whole cells. This in vitro assay employs the quenched substrate Rhodamine 110-(RR)2, which fluoresces green upon cleavage by active cathepsin enzymes. Analyze samples using flow cytometry or fluorescence microscopy.
lyophilized powder, 10 units/mg solid
sufficient for 200 fluorometric tests
For screening of hyaluronidase inhibitors, and quantification of hyaluronidase activity in cells and other biological fluids.
Combining the sensitivity of a fluorescence-based assay with a microplate format enables a rapid, quantitative readout suitable for high-throughput analysis. In a microplate well, the fluorescent signal can be generated within whole cells, in cell lysates, or in purified enzyme preparations and may then be analyzed by measuring fluorescence intensity from the well without the need for cellular imaging.
In diagnosis of Rheumatoid Arthritis (RA), the measurement of anti-CCP is integral. People who test positive for Anti-CCP, are very likely to develop or have already developed RA, but not all who have RA, necessarily develop anti-CCP. Early detection of RA is crucial for reducing tissue damage.
Key features and details Assay type: Quantitative Detection method: Colorimetric Platform: Microplate reader Assay time: 30 min Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine Sensitivity: 0.02 mM
An acetyl-biotinylated substrate recognized by a Eu3+ anti-acetyl MAb and SA-XL665, which is used in activator screening or to test an High Throughput SIRT1 inhibitor. The level of enzymatic activity is inversely proportional to the amount of signal produced in the system. For more information please visit Cisbio Bioassays website.
