Green Cathepsin B Assay
ICT’s Green Cathepsin B Assay allows researchers to detect and monitor intracellular cathepsin activity over time in vitro using live whole cells. This in vitro assay employs the quenched substrate Rhodamine 110-(RR)2, which fluoresces green upon cleavage by active cathepsin enzymes. Analyze samples using flow cytometry or fluorescence microscopy.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Cathepsins are a group of protease enzymes typically found in the lysosome. Cathepsin B plays important roles in many cellular functions (like intracellular protein degradation, antigen processing, etc.). Elevated cathepsin activity is associated with many disease states such as cancer, Alzheimer’s disease, and so on.
The Green Cathepsin B Assay contains Rhodamine 110 Cathepsin B Substrate, which is a non-cytotoxic and cell membrane permeant substrate that fluoresces green upon cleavage by active cathepsins. To use Rhodamine 110-(RR)2, add the substrate directly to the cell culture medium, incubate, and analyze. Because the substrate is cell permeant, it easily penetrates the cell membrane and the membranes of the internal cellular organelles – no lysis or permeabilization steps are required. If active cathepsin enzymes are present, the quenched Rhodamine 110-(RR)2 substrate is cleaved, resulting in an increase in green fluorescence signal. Samples can be analyzed by flow cytometry or fluorescence microscopy. Hoechst 33342 is included in the kit to label nuclei.
