Cell-Based Assays > Cell Signaling Assays Products & Reviews

High-throughput rapid detection of Luciferase activity

Millipore's full-length mTOR kinase is now available as part of the KinaseProfiler™ Service. This kinase is the first biologically relevant mTOR to be commercially available. The KinaseProfiler Service offers 264 kinases for selectivity profiling, using a catalytic radiometric assay format. The addition of the full-length mTOR to the KinaseProfiler panel provides high-quality, confidential screening of compounds against this c…

Cisbio offers a line of three ready-to-use assays for measuring cAMP using HTRF® technology. Each kit is optimized for detecting a specific range of cAMP concentrations to allow fine tuning of assay sensitivity. These kits enable high quality, cell-based assay screening in 96-, 384- or 1536-well formats.

Stable, recombinant AequoScreen ® parental cell line expressing aequorin and Gα16 in CHO-K1 host cells.

Real-Time qPCR assays for kinases and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Real-Time qPCR Case, Immersion Fluid, Frame Adhesive and Plate-File CD.

Real-Time qPCR assays for Signal Transduction and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Real-Time qPCR Case, Immersion Fluid, Frame Adhesive and Plate-File CD.

R&D Proteome Profiler Antibody Arrays R&D Proteome Profiler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment, and eliminate the need for multiple immunoprecipitation/Western blot experiments. R&D Array kits contain buffers, detection antibodies, and membranes spotted in duplicate with capture antibodies carefully selected for their specificity. R&D Arrays…

R&D Proteome Profiler Human Phospho-Kinase Array Kit - Human Phospho-Kinase Array Kit for the parallel determination of the relative levels of protein phosphorylation. The kit contains 4 sets of 2 membranes - each spotted in duplicate with 46 different kinase antibodies. The R&D Human Phospho-Kinase Array is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of phosphorylation of 46 kinase ph…

Cisbio Bioassays has developed Cellul'erk, a highly accurate HTRF assay for measuring phosphorylated ERK1/2 in 96-, 384- and 1536-well formats providing for an HTS compatible assay. Features: • Simple assay protocols designed for research studies (96-well plates) for HTS (384-small vol. plates) • Cell-based functional assay • Antibody-based detection • Direct detection Applications: • Gi, Gs, or Gq coupled receptor activatio…

G-Protein Coupled Receptors (GPCRs) are common drug targets. Previously, most studies were based on the monomeric model, but this perception of GPCRs is now evolving towards homodimeric , heterodimeric and oligomeric models. To facilitate research into GPCR dimers, CisBio Bioassays has applied the Tag-lite® technology to GPCR interaction assays. Based on HTRF® technology, plasmids have been designed to express either the SNA…

Cisbio Bioassays offer a comprehensive cellular platform to develop HTRF® ligand binding assays.Features: • Single technology and cellular platform for all GPCR assays • Unique non-radioactive ligand binding assays • Miniaturization down to < 10µl • Peptidic and non-peptidic fluorescent ligands • High Specific Binding • Low Non-Specific Binding with HTRF technology Tag-lite fluorescent ligands available for (Feb10): Vasopre…

Components of the Upstate (Millipore) Casein Kinase 2 Assay Kit:• Casein Kinase 2 Substrate Peptide (Cat.# 12-330) • Assay Dilution Buffer I (ADBI) (Cat.# 20-108) • PKA Inhibitor Cocktail (Cat.# 20-114) • Magnesium/ATP Cocktail (Cat.# 20-113) • P81 Phosphocellulose Squares (Cat.# 20-134) For use to measure the phosphotransferase activity of Casein Kinase 2 (CK-2) in immunoprecipitates and column fractions based on phosphorylat…