ADME-Toxicology > Cell Death / Health Assays Products & Reviews

Multiplate live-cell analysis system

The ApoTox-Glo™ Triplex Assay combines three assay chemistries to easily assess viability, cytotoxicity and apoptosis events in the same cell-based assay well. First, viability and cytotoxicity are determined by measuring two differential protease biomarkers simultaneously with the addition of a single nonlytic reagent containing two peptide substrates. The live-cell protease activity is restricted to intact viable cells and i…

The Griess Reagent System measures nitrite (NO2–), which is one of two primary stable and nonvolatile breakdown products of nitric oxide (NO). Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879. Through the years, many mod…

The CellTox™ Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended to assess cytotoxicity in cell culture after experimental manipulation. The assay system uses a proprietry asymmetric cyanine dye that is excluded from viable cells but preferentially stains the DNA from dead cells. When the dye binds DNA released from cells, its fluorescence properties are s…

The CellTiter-Glo® 3D Cell Viability Assay is a homogeneous method to determine the number of viable cells in 3D cell culture based on quantitation of the ATP present, which is a marker for the presence of metabolically active cells.   This ready-to-use reagent is based on the original CellTiter-Glo® Luminescent Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when prepa…

A Fluorometric Method for Estimating the Number of Nonviable Cells Present in Multiwell Plates

Digitonin can be used in cell viability and cytotoxicity assays. It is a detergent solution useful for permeabilizing cells and for creating a cytotoxicity chemistry positive control. Supplied at 20mg/ml in DMSO

LDH-Glo™ Cytotoxicity Assay detects cell death from a small number of cells, including 3D cell cultures, based on the release of lactate dehydrogenase.

The RealTime-Glo™ MT Cell Viabiilty Assay measures viability of cells in a non-lytic, real-time live-cell format, saving time, cells and reagent costs.

The RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay provides a no-wash, one-step Annexin V assay that allows continual monitoring of cell state to determine apoptotic onset using a plate-reading luminometer.

The Caspase-Glo ® 3/7 Assay System is  a highly sensitive caspase activity assay for use in plate-reader assays.

The Caspase-Glo ® 1 Inflammasome Assay  directly measures inflammasome activation with a simple add-mix-measure protocol.

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of action of antibodies through which virus-infected or other diseased cells are targeted for destruntion by components of the cell-mediated.

The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultur…

The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96® AQueous One Solution Reagent contains a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS(a)] and an electron coupling reagent (phenazine ethos…

The Promega CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The CytoTox 96® Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that result…

ADAM SCC2, a fast, easy and accurate automated somatic cell counter, is composed of fluorescence optic and image analysis software. It can be useful equipment for dairies, smaller labs and veterinary establishments working with somatic cell analysis. It is helpful to the implementation of milk quality control.

ADAM SCC is fully compatible with the Direct Microscope Somatic Cell Count (DMSCC) with the unique image cytometer technology.

We offer extensive Antibody Functional Characterization Services, focusing on both Fab-based target binding and Fc-mediated effector functions. Our assays analyze mechanisms like Neutralization, ADCC, CDC, and ADCP, leveraging advanced platforms to select promising therapeutic candidates.

ARRALYZE 8 Chamber Slide combines standard slide format with advanced nanowell technology, featuring 8 chambers with 127 precisely etched nanowells (0.4 × 0.4 × 0.225mm) per chamber. The borosilicate glass bottom (0.175μm thick under wells) provides superior optical clarity for high-resolution imaging while maintaining an inert environment. Includes 3 fiducials per chamber for easy navigation and precise cell tracking.

The only kit you’ll need to build your own organ-on-chip

Kits to covalently label dead cells, allowing cells with permeable plasma membranes to be excluded from analysis in flow cytometry. A wide variety of dye options for standard flow, spectral flow, and fluorescence microscopy.

The open organ-on-chip platform

The ApopTag ISOL Fluorescence Apoptosis Detection (DNase Types I & II) technique is based upon the biochemical specificity of the enzymes T4 DNA ligase and Vaccinia Virus DNA Topoisomerase I and the unique, dual labeled, dual hairpin oligonucleotide

The CHEMICON APO-DIRECT™ Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.