PCR and Thermal Cycling Products & Reviews

Thermo Scientific Arktik Thermal Cycler is suitable for a wide range of applications from individual reactions to high-throughput projects. It offers flexibility with three different interchangeable blocks: 96-well block, 384-well block, and a dual block with two 48-well units. A broad temperature gradient in 96 and 384 blocks simplifies temperature optimization. Ease-of-use is the key feature of the Arktik Thermal Cycler’s us…

Introducing the LightCycler® 96 Real-Time PCR System What if you had the power to get your qPCR data fast, reliably and with less effort? Well, now you can. The result of more than a decade of Roche experience developing high-performance qPCR systems is now accessible for any research lab. The LightCycler® 96 System delivers data that makes you a publication superhero: • Fast precision thermal cycling • Leading-edge optics fo…

Syzygy FlashTaq 2x Master Mix is a ready-to-use 2x mixture of FlashTaq, dNTPs, buffers and magnesium for efficient PCR amplification. Just add template DNA, primers, and water. FlashTaq Hot-Start DNA Polymerase is a chemically modified Taq polymerase designed for reducing nonspecific DNA amplification including primer dimers. The activiation time for FlashTaq is only 2 minutes at 95°C, which is faster than that for leading a…

rEVAlution 2X qPCR Master Mix is a ready to use hot-start qPCR master mix with EvaGreen® and FlashTaq, a hot start Taq polymerase. A 25μM ROX reference dye is supplied separately for use with some real time instruments. The master mix is optimized to work with both fast cycling conditions and regular three step cycling conditions. Available in 3 pack sizes: 100 20µL Reactions, 500 20µl reactions and 1,000 20µl reactions.

PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR. Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension,…

Primer and probe premixed assays for analyzing gene expression in any species using fluorescently labeled 5' nuclease probes

LightCycler® Nano Instrument - Real-time PCR amplification and detection instrument for tube-based assays of up to 32 (4 x 8) samples per run, with minimal noise, footprint and weight. Assembled from just a few building blocks, the robust LightCycler® Nano System is easy to transport and install. Up to 32 samples can be run in 0.2 ml tubes connected as strips. Fast heating and cooling is done using Peltier elements. Assay per…

Rapid and accurate detection of foodborne pathogens

The Thermo Scientific* SureTect Listeria monocytogenes PCR Assay is a multiplex real-time assay that incorporates unique primer and probe components for highly-specific detection of the prfA gene, specific to and found only in Listeria monocytogene.The assay also includes primer and probe components, for simultaneous detection of an internal amplification control (IAC), for confirmation of successful PCR and increased confiden…

Obtain high quality results with this thermostable probe-based qPCR master mix

1 ml, one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 100 x 20 µl reactions

100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. The Phire® Animal Tissue Direct PCR Kit employs Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA-binding domain that enhances the processivity of the polymerase. Phire Hot Start II DNA Polymerase also exhibits extreme…

Thermo Scientific Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to PCR inhibitors present in blood and retains its activity even with samples containing 40% whole blood. This extremely accurate proofreading DNA polymerase makes the Phusion B…

Pre-designed, gene-specific probe and primer pairs for repeatable, sensitive and gene specific RT-qPCR quantification for human and mouse protein-coding genes and long noncoding RNAs (lncRNAs). Thermo Scientific Solaris qPCR Gene Expression Assays are pre-designed, gene specific probe and primer pairs that utilize minor groove binder (MGB) and Superbase technologies to deliver repeatable, sensitive and gene specific RT-qPCR (…

2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions

The Labcycler has a processor controlled block with separately controlled peltier elements for extraordinary temperature homogeneity at high heating and cooling rates. State-of-the-art peltier elements have demonstrated more than 600000 cycles so far without any failures. This gives more than 15 years of lifetime even under the most demanding conditions, like DNA-sequencing on a daily basis. Two Labcycler can be connected with…

SensiFAST™ cDNA Synthesis Kit has been developed to generate high-quality first strand cDNA from total RNA template. SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first strand cDNA synthesis for use in real-time PCR studies (fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, reg…

SensiFAST™ Probe Hi-ROX Kit and SYBR® Hi-ROX Kit have been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Kits delivers fast, highly-specific and ultra-sensitive real-time PCR.SensiFAST™ Probe Hi-ROX Kit has been d…

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 200 x 20 µl reactions

Clever and unique automation that speaks PCR Setup! Automated liquid handling and dedicated PCR setup software, which is optimized for touch screen operation, create the ideal fusion for your OEM PCR Setup Solution. Combined with your PCR reagents this new solution creates a competitive package, where the system leaves you enough flexibility to compose the system the way you need it for your kits. The PCR Setup Solution is d…

Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System

Our Flipper® racks present colorful solutions and space saving utility for tubes ranging in size from 0.2mL all the way up to 50mL. Available in different configurations to meet laboratory needs.

Precision Blue™ Real-Time PCR Dye, 55 µl (500 x 20 µl reactions)