CombiMag improves your transfection reagent efficiencyCombiMag reagent has been designed to be employed in association with all transfection reagents complexed with any types of nucleic acids. All cell types, even hard-to-transfect and primary cells, can be transfected using CombiMag associated to any working reagent, even if its original efficiency is low.CombiMag has been associated with many existing transfection reagents i…
FlyFectin ™ is a specifically designed and very efficient transfection reagent for insect cells. Innovative and unique, FlyFectin ™ is: • Highly efficient with excellent reproducibility• Multipurpose (various types of nucleic acids)• Ideal for recombinant protein production with Baculovirus expression system• Simple, Ready-to-use and Rapid• Compatible with and without serum-containing culture media• Non toxic• Economi…
B-Pak, is a straightforward and rapid procedure for determining the concentration of protein in solution. Thanks to an optimized Bradford reagent formulation, B-Pak gives precise determination of protein concentration even in the presence of detergent, allowing the use of this kit for transfection experiments. In addition, this kit can be used for all protein concentration measurement due to a superior linearity of response: f…
An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…
High productivityVersatile: efficient in both HEK 293 & CHO synthetic mediaComplies with new regulatory requirements for large scale production
OnTarget™ is a powerful, new platform for detection of rare tumor mutations in tissue or plasma. • Detect 100 or more mutations in a single assay, without sample splitting • Quantify mutations present at 0.01% allele frequency and above • Enrich mutations extracted from plasma or FFPE in about 1 hour
High efficiency electroporation using any instrumentElectroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity. Ingenio Kits and Solution are compatible with multiple conventional electroporation instruments including Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser and Harvard-BTX® electroporators.Ingenio® Cuvettes Features:•High effi…
High efficiency electroporation using any instrumentElectroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity.Ingenio® Cell Droppers Features:•High efficiency electroporation of hard to transfect cell lines and primary cells•Compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®•Idea…
100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water
Efficient siRNA or microRNA transfection. To attain efficient and reliable siRNA or microRNA transfection, we offer DharmaFECT transfection reagents. Thermo Scientific DharmaFECT 1 is our most all-purpose transfection reagent formulation; demonstrating efficient, low-toxicity delivery to over 35 cell types. DharmaFECT 1 achieves effective transfection of siRNA and microRNA reagents at low concentrations and with low cytotoxic…
SmartRNAplex miRNA Assays are your benchtop solution for miRNA profiling, using a familiar protocol, and simple acquisition software. SmartRNAplex microRNA Assays use proprietary Firefly™ technology, which exploits uniquely encoded hydrogel particles developed by innovators at Firefly BioWorks. The Firefly™ hydrogel particles are hydrophilic, bio-inert, flexible and porous, allowing target molecules to diffuse and bind in a…
Research and Clinical Grade RetroNectin Reagent for Retroviral or Lentiviral Transduction. RetroNectin Reagent for retroviral or lentiviral transduction significantly increases transduction efficiency and results in enhanced gene transfer, especially in suspension and hematopoietic stem cells. RetroNectin Reagent is a chimeric peptide composed of E. coli-derived recombinant human fibronectin fragment that contains three funct…
Mouse ROSA26 safe harbor gene knock-in kit and clones. ROSA26 (also known as ROSAβgeo26 locus) in mice genome is first found in chromosome 6 in one particular strain of mice-named ROSAβgeo26-expressed β galactosidase from a randomly inserted transgene at high levels uniformly in nearly all tissues examined. This locus expresses one coding transcript and two noncoding transcripts, and only the non-coding transcripts are disrup…
The DNA Ligation Kit Mighty Mix is a single solution, ready-to-use 2X ligation mix that enables high efficiency ligations, particularly for blunt-ended ligations. This DNA ligation kit can also be used for ligation into TA-vectors. The basic ligation reaction can be quickly performed, either for 5 minutes at 25°C or for 30 minutes at 16°C, with equal results for both circular and linear DNA ligations. The 2X Ligation Mix is c…
GeneCopoeia offers complete custom services for CRISPR-Cas9-based targeted genomic modification, including CRISPR design and construction, donor design and construction, functional validations, stable cell line establishment, and transgenic mouse generation. Service Details: Validation Services Donor clone services Stable cell line services Transgenic mouse services Advantages: Fast delivery of CRISPR-Cas9 plasmids…
SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first strand synthesis, without adaptor ligation. The presence of these known sequences is crucial for a number of downstream applications including amplification, RACE, and library construction. While a wide variety of technologies can be employed to take advantag…
With over 20 million pre-made clones representing nearly every known gene for most common organisms, Source BioScience are your perfect partner for quick and easy solutions for gene and protein expression research. Source BioScience customers can choose from individual pre-made clones or entire clone libraries from many of the most well-known and cited clone consortia and research groups for mammalian and non-mammalian specie…
Source BioScience are a trusted provider of outsourced services for gene expression projects. Source BioScience work with three major microarray platforms (Affymetrix, Agilent and Illumina) and optimise the most suitable array technology for each project.
Takara's DNA Ligation Kit for Long Fragments is a powerful tool for the cloning of long DNA fragments spanning 2 kb to over 10 kb in length. The kit contains an optimized ligase/buffer system that enables ligation of long fragments without the need for extensive techniques or special expertise. It is especially well suited for BAC library construction. Features: Superior ligation efficiency, especially for long DNA frag…
Roche FuGENE HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. Roche FuGENE HD Transfection Reagent is designed to transfect a wide range of eukaryotic cells, including insect cells and many tumor cell lines not transfected well by other reagents (e.g., MCF7, Hep G2, PC-3, HeLa, MA-10, Hep G2, SH-SY5Y, A7r5, STO, SCC-61, SQ20B, T98G, STSAR-90, A375, A549, and others). In addi…
Protein Analysis Software - the Next GenerationCLC Protein Workbench creates a software environment enabling users to make a large number of advanced protein sequence analyses, combined with smooth data management, and excellent graphical viewing and output options. Protein Workbench sets new standards for bioinformatics software with its overall graphical display, usability driven and user-friendly interface, while featuring…
Free Bioinformatics Software Not a demo. Not scaled down. No spyware. No need to register. Yet still a wide variety of features available - and - free! CLC Free Workbench creates a software environment enabling users to make a large number of bioinformatics analyses, combined with smooth data management, and excellent graphical viewing and output options. Some of the many bioinformatics analyses: • Interactive restriction site…
The New Standard for Desktop-Based Bioinformatics Tools CLC DNA Workbench provides a software environment which enables users to perform advanced DNA sequence analyses such as assembly of DNA sequence data, graphically and algorithmically advanced primer design, while offering user-friendly molecular cloning tools. CLC DNA Workbench is a bioinformatics software package containing a range of specialized DNA analyses and bioinfo…
Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. Features: High yields of capped RNA—up to 39 µg of RNA per reaction Extra convenience—cap analog-nucleotide mix provided Simplified rea…
The MycoAlert™ PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlert™ PLUS Substrate, c…
