FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results thanks to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incubation step at 95°C. PCR Hot Start PCR RT-PCR Benefits Insist on higher specificity, sensitivity…
The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR-Grade Nucleotides.FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incuba…
The enzyme dNTPack comprises the FastStart High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides. The FastStart High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb. Hot Start PCR Multiplex PCR RT-PCR GC-rich template amplification Difficult…
The FastStart High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb. Hot Start PCR Multiplex PCR RT-PCR GC-rich template amplification Difficult, challenging PCRs The FastStart High Fidelity PCR System is the product of choice for multiplex PCR. Please see the onl…
The enzyme dNTPack comprises the GC-RICH PCR System and a ready-to-use solution of PCR-Grade Nucleotides.The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior perfo…
The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates. PCR Amplification of difficult templates up to 5 kb…
The enzyme dNTPack comprises the Expand High FidelityPLUS PCR System and a ready-to-use solution of PCR-Grade Nucleotides. Expand High FidelityPLUS PCR System is a next-generation blend consisting of Taq DNA Polymerase and a novel proofreading protein, isolated and characterized by Roche Applied Science. This protein mediates proofreading activity but has no polymerase activity itself. Choose Expand High FidelityPLUS PCR Syste…
Upgrade from the Expand Long Template PCR System and choose the new Expand Long Range dNTPack for consistent amplification of long PCR products. Generate PCR products of 5 to 25 kb from genomic DNA with this next-generation system from the company that pioneered long-template PCR. This specially designed PCR system combines a single buffer set for all product sizes, ultrapure PCR-grade nucleotide mix, and an optimized blend of…
The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length. PCR RT-PCR Benefits The E…
The enzyme dNTPack comprises the Expand High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides. The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR…
The enzyme dNTPack comprises the Pwo SuperYield DNA Polymerase and PCR-Grade Nucleotides in a ready-to-use solution.Pwo SuperYield DNA Polymerase is ideal for high-fidelity amplification of DNA up to 3 kb. Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. This new buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of…
The kit is designed for the preparation of total RNA mini-preps (free of contaminating DNA) from cultured cells. Other sample materials like blood, yeast, and bacteria require a pre-lysis treatment. The purified and intact RNA is of high quality, and is suitable for RT-PCR and northern blotting, RNase protection, or primer-extension experiments. Up to 24 samples can be simultaneously processed in approximately 1 hour. Benefit…
The High Pure RNA Tissue Kit is designed to purify total, intact RNA from tissue samples (e.g., mouse liver, spleen, lung, heart) for use in research. The isolated RNA may be used directly for RT-PCR, northern blotting, and RNase protection assays. PrincipleAs a prerequisite for the reverse transcription polymerase chain reaction (RT-PCR), intact RNA must be isolated from tissue. Tissue samples are homogenized in the presence…
Isolate total RNA from formalin-fixed, paraffin-embedded tissue as well as fresh-frozen, tissue-research samples for use in RT-PCR with the High Pure RNA Paraffin Kit. The quality of RNA obtained with this kit is suitable for relative quantification of mRNA with RT-PCR, especially with the LightCycler® Carousel-Based System. Benefits Can isolate RNA suitable for RT-PCR from archived paraffin tissue (up to 15 years old). Yi…
Use the High Pure 96 UF Cleanup Kit to purify amplification products for direct use in applications such as microarray spotting or sequencing. The rapid protocol allows simultaneous purification of 96 reactions in less than 20 minutes with excellent recovery. Benefits Choose a flexible format. Samples can be processed manually using vacuum manifolds or microplate centrifuges, or using an automated method on standard liquid-…
The kit is designed for the efficient and convenient isolation of PCR products from amplification reactions. Primers, mineral oil, salts, unincorporated nucleotides, and the thermostable DNA polymerase may inhibit subsequent enzymatic reactions (e.g., labeling, sequencing or cloning of the PCR products). In addition, nucleic acids from other modification reactions (e.g., restriction-endonuclease digests, alkaline-phosphatase t…
The High Pure Viral RNA Kit is intended for general laboratory use and is designed to purify viral RNA from serum or plasma. Viral RNA is used for RT-PCR analysis directly after elution in nuclease-free water. Principle As a prerequisite for the reverse transcription polymerase chain reaction (RT-PCR), viral RNA must be isolated from serum or plasma. Virus lysis is accomplished by incubation of the sample in a special Lysis/Bi…
The High Pure Nucleic Acid Kit is intended for general laboratory use and is designed to purify viral nucleic acids from serum, plasma, or whole blood, using sample volumes of up to 200 µl. When using whole blood, total nucleic acids are isolated, including viral nucleic acids. The purified viral nucleic acid is used for PCR and RT-PCR analysis directly after elution in nuclease-free water. PrincipleAs a prerequisite for PCR o…
This kit is used to prepare purified plasmid DNA in small quantities, known as “mini-preps”, using the alkaline lysis method, a commonly used method that generates highly purified plasmid DNA from E. coli, free of RNA contamination. The kit is designed for the isolation of up to 10 µg of plasmid DNA from 0.5 to 4 ml of bacterial suspension. Up to 24 samples can be processed at the same time in less than 45 minutes. The purifie…
This kit is designed to purify nucleic acids from different sample materials, including whole blood, cultured cells, and tissue samples. Bacteria and yeast require a specific prelysis treatment with lysozyme or lyticase. The resulting nucleic acids are ready for use in PCR and restriction-digestion reactions. Benefits Maximize flexibility in sample preparation. This versatile kit works well with a wide variety of sample ma…
Use the High Pure FFPE RNA Micro Kit to isolate total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples for direct use in RT-PCR. The isolated RNA is suitable for relative quantification of mRNA in RT-PCR using real-time PCR systems such as the LightCycler® Carousel-Based System and the LightCycler® 480 System. Isolate total RNA from 1 to 10 µm sections of formalin-fixed, paraffin-embedded tissue (e.g., colon, b…
The Genopure Plasmid Midi Kit is used to prepare purified plasmid DNA in medium quantities known as “midi preps”. Using a modified alkaline lysis method, highly purified plasmid DNA from E. coli (free of RNA contamination) is generated. The kit is designed for the isolation of up to 100 µg of plasmid DNA from bacterial culture. Depending on the number of plasmids, use a 5-ml to 30-ml (high-copy number) or 10-ml to 100-ml (low-…
The Direct Sample Probe system for the Finnigan TRACE DSQTM, Finnigan FOCUS DSQ, and Finnigan PolarisQ is ideal for qualitative or semi-quantitative analysis of materials that are difficult, if not impossible to elute chromatographically.Product detail:The Direct Sample Probe system for the Finnigan TRACE DSQTM, Finnigan FOCUS DSQ, and Finnigan PolarisQ is ideal for the quick screening of samples or for any application which d…
The new Finnigan LCQ Advantage MAX delivers high productivity LC/MS/MS analysis in a routine HPLC environment. Product detail: The Finnigan LCQ Advantage is the workhorse ion trap mass spectrometer for high productivity LC/MS/MS results and is easily upgraded to MSn performance. The Finnigan LCQ Advantage MAX now features the universal Ion Max source that allows simple tool-less switching of ionization probes. Ion Sweep Gas…
The MAT 95 XP is a highly flexible GC-MS system fully automated regardless of injector type and offers multicolumn operation as a standard feature. Autotuning assures optimized instrument performance with minimal user interaction at any resolution setting – fast, automated and reproducible, and only ONE calibration table making for simpler operation. Product detail: High mass resolution Accurate mass High sensitiv…
