High Pure RNA Isolation Kit
The kit is designed for the preparation of total RNA mini-preps (free of contaminating DNA) from cultured cells. Other sample materials like blood, yeast, and bacteria require a pre-lysis treatment. The purified and intact RNA is of high quality, and is suitable for RT-PCR and northern blotting, RNase protection, or primer-extension experiments. Up to 24 samples can be simultaneously processed in approximately 1 hour. Benefit…
Great!
Low to medium throuhgput, mini scale RNA isolation
The High Pure RNA Isolation Kit rapidly isolates small amounts of total RNA from a variety of samples, such as cultured cells, whole blood, yeast, and bacteria. The isolated RNA can be used in many downstream applications. Quickly process multiple samples
Review Date: 24 Oct 2017
The kit is designed for the preparation of total RNA mini-preps (free of contaminating DNA) from cultured cells. Other sample materials like blood, yeast, and bacteria require a pre-lysis treatment. The purified and intact RNA is of high quality, and is suitable for RT-PCR and northern blotting, RNase protection, or primer-extension experiments. Up to 24 samples can be simultaneously processed in approximately 1 hour.
Benefits
- Quickly process multiple samples.
A single sample preparation is completed in 25 minutes, and multiple samples can be prepared in 45 minutes. - Prevent RNA loss.
Precipitation or solvent extraction steps are not required. - Avoid DNA contamination.
Integrated DNA digestion and DNase removal eliminates interference from genomic DNA. - Obtain concentrated RNA.
RNA is eluted in a 50 µl volume.
Gene Expression Analysis Comparing Two RNA Isolation Methods from Recent and Older Archived Formalin-Fixed, Paraffin-Embedded Tissue (FFPET) Samples
Formalin-fixed, paraffin-embedded tissue samples are widely available for retrospective studies. However the quantity and the quality of the isolated RNA can limit downstream applications. In this application note the High Pure FFPET RNA Isolation Kit is compared with a commercially available FFPE RNA isolation kit with regard to the quality and quantity of the isolated RNA. It is demonstrated that high quality and quantity of total RNA can be isolated from FFPE blocks using High Pure FFPET RNA Isolation Kit, providing reliable starting material for gene expression studies.
