Worthington is a privately owned, ISO9001 certified, primary manufacturer of high-quality purified enzymes, proteins, nucleic acids and primary cell isolation kits for biotech and life science research, diagnostic, biopharmaceutical and bioprocessing applications. Our reputation for producing quality products for all types of life science applications is the result of over 60 years of experience. Worthington in-house, full-service capabilities include initial extraction, fermentation, protein purification, lyophilization and packaging. All processes are fully documented and traceable from raw materials through final shipment. All manufacturing and purification takes place at Worthington facilities located in Lakewood, New Jersey, USA. All animal-based raw materials supplied to Worthington are obtained from United States Department of Agriculture (USDA) or equivalent approved facilities, inspected and certified to be healthy, free of disease and suitable for human consumption and/or exportation. All slaughtered animals are given ante and post-mortem inspections and those that are found to be wholesome are passed for human consumption. Several animal origin free (AOF) enzymes are also produced and pending development by Worthington as an alternative to animal sourced products, thereby eliminating TSE/BSE and mammalian viral contaminant risks. We welcome the opportunity to discuss specific requirements and collaborative development projects.
Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types 1, 2, 3, and 4 or chromatographically purified collagenase (the latter usually combined with secondary enzymes such as elastase, hyaluronidase, etc). For best results, the precise mixture of proteolytic activities must be tailored to the tissue to…
