Promega Corp. Products & Reviews

Reliable Transfection for Common Cell Lines

Single-Use Ligands for High-Resolution lmaging in Live Cells

Capped or Uncapped RNA Transcripts for in vitro or in vivo Translation

Digitonin can be used in cell viability and cytotoxicity assays. It is a detergent solution useful for permeabilizing cells and for creating a cytotoxicity chemistry positive control. Supplied at 20mg/ml in DMSO

Extract high-quality nucleic acids with less hands-on time.

Rapid detection of firefly luciferase activity with amazing sensitivity and dynamic range.

High-Throughput Extraction of DNA from FFPE Samples Robust, precipitation-free protocol, no chance of lost pellets High yields of pure DNA from FFPE samples without using xylene or other hazardous chemicals Ideal for use in downstream applications including qPCR and next-generation sequencing (NGS)

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:…

Column-based method for rapid concentration and cleanup of DNA.

Column-based method for rapid concentration and cleanup of RNA.

LDH-Glo™ Cytotoxicity Assay detects cell death from a small number of cells, including 3D cell cultures, based on the release of lactate dehydrogenase.

The RealTime-Glo™ MT Cell Viabiilty Assay measures viability of cells in a non-lytic, real-time live-cell format, saving time, cells and reagent costs.

The RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay provides a no-wash, one-step Annexin V assay that allows continual monitoring of cell state to determine apoptotic onset using a plate-reading luminometer.

The GSH/GSSG-Glo™ Assay is a bioluminescent assay to detect and quantitate total glutathione ratios.

The Caspase-Glo ® 3/7 Assay System is  a highly sensitive caspase activity assay for use in plate-reader assays.

The Caspase-Glo ® 1 Inflammasome Assay  directly measures inflammasome activation with a simple add-mix-measure protocol.

Detect and quantify any tagged protein with a simple, luminescent signal.

Measures NanoLuc® Luminescence in Living Cells Substrate and buffer optimized to enhance stability Use to detect NanoBiT® protein complementation or NanoLuc® reporter activity Monitors luminescence at a single time point or continuously for up to 2 hours without compromising cell viability.

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of action of antibodies through which virus-infected or other diseased cells are targeted for destruntion by components of the cell-mediated.

Measure neonatal Fc receptor binding  

Optimized PCR master mix: Just add template, primers and water

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

New Site-Specific Endoprotease that Targets Proline and Alanine

MOA-based functional bioassay to measure Fc effector activity.

Stable at Room Temperature and Easy to Use