Lumit™ FcRn Binding Immunoassay
Measure neonatal Fc receptor binding
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- No wash or transfer: Simple add-mix-read protocol
- Results in less than one hour
- Scalable and high throughput: Perform assays in 96-well or 384-well plates
The Lumit™ FcRn Binding Immunoassay is a novel homogeneous (no-wash) competition assay to measure the interaction between human FcRn and Fc proteins, including antibodies. In the assay, a human IgG1 labeled with LgBiT (Tracer-LgBiT) is used as the tracer. A C-terminal biotinylated human FcRn bound to Streptavidin-SmBiT (hFcRn-Biotin-SA-SmBiT) is used as the target. In the absence of an antibody analyte sample, Tracer-LgBiT binds to the hFcRn-SmBiT target, resulting in maximum luminescence signal. In analyte samples, unlabeled IgG will compete with Tracer-LgBiT for binding to the FcRn target, resulting in a concentration-dependent decrease in luminescent signal.
