New England Biolabs Inc. Products & Reviews

The NEBNext® Ultra™ RNA Library Prep Kit for Illumina contains enzymes and buffers that are ideally suited for cDNA library preparation for next-generation sequencing. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must p…

The NEBNext Small RNA Library Prep Set for SOLiD™ contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the SOLiD platform. NEBNext Small RNA Library Prep Set for Solid is multiplex compatible. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next genera…

Restriction enzyme digests are now even easier!The same high quality restriction enzymes that you have come to trust from New England Biolabs are now available in master mix format, including enzyme buffer and loading dye; simply add your DNA and digest for 15 minutes.Available Mixes: AgeI-HF® RE-Mix® OrderAscI RE-Mix® OrderBstEII-HF® RE-Mix® OrderEcoRI-HF® RE-Mix® OrderEcoRV-HF® RE-Mix® OrderKpnI-HF® RE-Mix® Or…

Western blotting is a widely used technique to detect levels of protein expression in cell or tissue extract. This technique measures protein levels in a biological sample through antibody binding to a specific protein of interest.   Features: Detects Small Amounts of Protein – delivers a sensitive and robust signal for standard protein detection Convenient, Cost-effective 500 ml Sizing – means you can p…

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences

PvuII has a High Fidelity version PvuII-HF® (NEB #R3151) High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.  HF enzymes also exhibit dramatically reduced star activity.  HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without de…

The Quick Dephosphorylation Kit contains Quick CIP, a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.

The New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for autom…

An optimized blend of Taq and Deep Vent® DNA Polymerases, LongAmp® Taq DNA Polymerase enables amplification of up to 30 kb PCR products with a fidelity higher than Taq DNA Polymerase alone. LongAmp Taq is also available with Crimson tracking dye (NEB #M0326) in the reaction buffer.

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. Time-Saver™  qualified for digestion in 5-15 minutes High Fidelity (HF®) version available (NEB #R3189) supplied with CutSm…

Streptavidin Magnetic Beads are 1 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids.

The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.

α2-3,6,8,9 Neuraminidase A is a broad specificity sialidase, which cleaves linear and branched non-reducing terminal sialic acid residues from glycoproteins, glycopeptides, and oligosaccharides. It can be used for glycan analysis and characterization and intact glycoprotein remodeling, in vitro and in vivo. Recombinant enzyme with no detectable endoglycosidase or other exoglycosidases contaminating activities Acts on b…

DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

Purifies up to 100 µg of high quality total RNA from multiple sample types - using only one kit

Taq DNA Polymerase optimized for epigenetics analysis

Quickly and easily purify high quality DNA from PCR and other enzymatic reactions.

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. Working with uracil-…

Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Assembly and transformation in just under two hours Flexible sequence design (scar-less cloning) No PCR clean-up step required High transformation efficiencies for inserts up to 20 kb Easily adapted for multiple DNA manipulations, including site-directed mutagenesis

The EnGen sgRNA Synthesis Kit,  S. pyogenes  provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.

Quickly and easily purify DNA from agarose gels with high yields.

Gibson Assembly® allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.

Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies.  Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.  Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart™ Buffer (over 215 enzymes are available in the same b…

Restriction Enzyme 

For Research Use Only. Not for use in diagnostic procedures.