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EnGen sgRNA Synthesis Kit

New England Biolabs Inc.Available: Worldwide

The EnGen sgRNA Synthesis Kit,  S. pyogenes  provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.

New England Biolabs Inc.

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Description

The EnGen sgRNA Synthesis Kit, S. pyogenes provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.

  • Rapid generation of microgram quantities of sgRNA
  • Complete workflow of less than one hour
  • Simple protocol requires only a user-supplied ~55 nucleotide ssDNA target-specific oligonucleotide
  • For help with oligo design, try EnGen sgRNA Template Oligo Designer - now included in the NEBioCalculator® Tool
  • Compatible with EnGen Spy Cas9 NLS, EnGen Spy Cas9 Nickase, EnGen Spy dCas9 (SNAP-tag®)

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