ImmunoChemistry Technologies is not just a contract laboratory for protein chemistry. We’re your trusted supplier for apoptosis assays, ELISA detection reagents, and custom ELISA development and other protein chemistry-related services. Our mission is to advance scientific discovery by developing innovative products for apoptosis detection, cellular analysis, and immunoassay development. We offer novel apoptosis detection reagents to study various biological processes and cellular physiological status, including apoptosis, caspase activity, mitochondrial health, and necrosis. Our ELISA Solutions™ are specialized ELISA buffers and immunoassay components for assay development, specially formulated to improve assay performance at each step. Our in vitro apoptosis assays detect active caspases, cathepsins, serine proteases, and assess mitochondrial health in cultured cells. Our proprietary FLIVO in vivo apoptosis assays detect apoptosis in living animal research models. The fluorescence-based FLIVO tracers have been used to research chemotherapeutic efficacy, neurodegeneration, and eye disease, among many other applications. One day, the apoptosis detection technology may be used to better manage disease and personalize treatment. FLIVO® won LifeScience Alley's New Technology Showcase Award in December 2008. Our current research activities are developing additional apoptosis probes for noninvasive imaging applications. ImmunoChemistry Technologies' contract laboratory specializes in custom ELISA development, assay manufacturing, testing, and protein chemistry-related services. From our experience performing these assay development services, ICT has developed and offers a specialty line of ELISA buffers and stabilizers for assay development and IVD test manufacturing.
ICT’s Green Cathepsin B Assay allows researchers to detect and monitor intracellular cathepsin activity over time in vitro using live whole cells. This in vitro assay employs the quenched substrate Rhodamine 110-(RR)2, which fluoresces green upon cleavage by active cathepsin enzymes. Analyze samples using flow cytometry or fluorescence microscopy.
Neptune™ HRP Conjugate Stabilizer preserves the activity of HRP-protein conjugates. This HRP stabilizer can be used to store concentrated stock HRP conjugates, reconstitute lyophilized conjugates, and dilute HRP conjugated antibodies to their useful working titer in ELISA tests and immunology-based techniques.Neptune™ HRP Conjugate Stabilizer is especially suited for ELISAs using anti-IgG HRP conjugates within traditional ant…
General Block contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide or protein antigen-down ELISA formats. General Block contains a mammalian protein blocking agent designed to reduce nonspecific background noise in most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. This unique, low-level blocking buffer minimizes nonspecific binding interacti…
Neptune Block Blocking Buffer is designed primarily for antigen-down ELISA formats as well as sandwich assays with high background problems. Neptune Block ELISA Blocking Buffer is particularly useful when working with human and other mammalian serum samples as it works to reduce interactions between sample and blocking molecules.Neptune Block ELISA Blocking Buffer is a mixture of small molecular proteins and molecular stabili…
Eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. SynBlock ELISA Blocking Buffer provides superior background-blocking performance without the use of conventional cross-reactive protein additives. SynBlock ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with…
Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs.Phosph-Free Block is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. However, it is appropriate for assays using HRP detection as well. Using inert no…
Reduce non specific binding in ELISAs and stabilize proteins with Alternative Block, an all-synthetic ELISA blocking buffer free of proteins and detergents. Alternative Block is a protein-free, detergent-free ELISA blocking buffer. This unique blocking buffer contains a heterogeneous mixture of proprietary blockers and synthetic stabilizers that block the uncoated regions of the microtiter plate without the use of conventiona…
Reduce non specific binding in ELISAs, address high background issues, and stabilize proteins with Monster Block, a new ELISA blocking buffer utilizing a heterogenous mixture of nonmammalian blocking agents. Monster Block™ provides a high degree of blocking efficiency and protein stabilization through the use of non-mammalian protein blocking agents and stabilizers. The heterogeneous composition of this unique ELISA blocking…
Detect caspase-1 activity with the FLICA® Caspase 1 Assay Kit. This in vitro assay employs the green fluorescent inhibitor probe FAM-YVAD-FMK to label active caspase 1 enzyme in living cells or tissue samples. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or by flow cytometry. Caspase 1 (ICE):Members of the mammalian caspase family of cysteinyl aspartate-specific proteases play disti…
Magic Red™ Caspase 3 and 7 Assay is a substrate-based fluorescent apoptosis assay that measures DEVDase enzyme activity in living, intact cells. Detection of caspase 3 and 7 activity (DEVDase enzymes) is a reliable method for assessing apoptotic activity in experimental cell populations. The Magic Red™ caspase 3 and 7 fluorogenic substrate, MR-(DEVD)2, is primarily targeted by caspases 3 and 7 of the apoptotic protease casca…
