The widespread use of human induced pluripotent stem cell (hiPSC) derived mature cell types has been restricted by complex and suboptimal differentiation protocols. bit.bio novel cellular reprogramming approach supported by a uniquely engineered genetic switch, opti-ox, is able to largely overcome these restrictions. This proprietary technology enables homogeneous and efficient differentiation of entire hiPSC cultures through tightly controlled expression of selected transcription factors. opti-ox reprogramming enables the consistent manufacture of homogenous and mature hiPSCs derived functional cells within days, offering access to the highest quality cellular models with simple protocols. ‘bit.bio’ represents the two fields — coding and biology — that determine the Identity of every human cell. Ultimately, bits are the building blocks of code, just as cells are the building blocks of life. This is reflective of what Bit Bio does: precise reprogramming of human stem cells. Our mission and focus are to enable a new generation of therapies through democratising access to consistent and functional human cells. This will improve research and drug discovery, lower the cost and extend the application of cell therapies.
A highly defined, scalable, and consistent source of human central nervous system (CNS) neurons. ioGlutamatergic Neurons, part of the bit.bio ioCells portfolio, are reprogrammed from human induced pluripotent stem cells (iPSC) using bit.bio's precise reprogramming technology: opti-ox™ (optimised inducible overexpression).
Human iPSC-derived glutamatergic neurons expressing Cas9 for rapid gene knockout generation
Human iPSC-derived microglia expressing Cas9 for rapid gene knockout generation
Introducing highly-defined, consistent, and reliable human muscle cells for research, disease modeling, and high throughput screening across areas such as muscle, neuromuscular, and associated metabolic disorders. ioSkeletal Myocytes, part of the bit.bio ioCells portfolio, have been reprogrammed from human induced pluripotent stem cells (iPSCs) using bit.bio's precise reprogramming technology: opti-ox™
A highly defined, scalable, and consistent source of human central nervous system (CNS) neurons carrying the disease-relevant 50 CAG trinucleotide repeat expansion associated with Huntington’s disease. ioGlutamatergic Neurons HTT 50CAG/WT have been reprogrammed from human-induced pluripotent stem cells (iPSCs) using bit.bio’s precise reprogramming technology: opti-ox™ (optimized inducible overexpression).
ioMicroglia are human induced pluripotent stem cell (iPSC)-derived microglia, precision reprogrammed using opti-ox™ technology. Within 10 days post-revival, ioMicroglia are ready for experimentation, expressing (>90%) key markers, including TMEM119, P2RY12, and IBA1. ioMicroglia display key functional traits including phagocytosis and proinflammatory cytokine secretion and can be co-cultured with ioGlutamatergic Neurons™.
ioSkeletal Myocytes DMD Exon Deletion disease models are built to help improve the translatability of data generated during the discovery of new candidate drugs for Duchenne muscular dystrophy.
