OT-2
Lab automation has never been easier. The OT-2 can automate hundreds of life science protocols and workflows. If you can manually pipette it, you can automate it with OT-2
The OT-2 in the Lab
OT-2 with Protocol Designer
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Good, basic pipetting robot
Broth microdilution
The protocols were relatively easy to run, though often a restart was needed each time a protocol was changed or re-run. Creating newprotocols was quite simple too. Even after calibration, the pipetting was unfortunately not accurate enough to use for our intended use.
Review Date: 21 Nov 2023 | Opentrons
Perfect to save your time for standard pipetting tasks
Automating pipetting protocols for cell culture experiments.
The price performance ratio is great! It is a simple and comparably cheap liquid handler which is capable of using two different pipette heads at a time. Pipetting volumes range from 20uL to 1000uL with single- or multi-channel heads. Multi-channel means it has 8 pipettes which cannot move independently. The programming is easy if you are already familiar with python. I think they also provide a non-programmer interface in which you can build your protocol. The support is usually targeting you to the guides that are available online, so rarely helpful cause I've read them already multiple times. However, the responding time is usually very fast. Currently, the company is releasing the next model of the robot, called Opentrons FLEX, which has even more functionality like automated calibrating. I think this is also the reason why the software maintenance of the old models got left behind a bit. I would be really careful with upgrading the server versions, cause the latest versions are sometimes buggy or are missing functions that were available beforehand. The server versions 4.7 to 6.0 are working quiet stable in my opinion.
Review Date: 27 Sept 2023 | Opentrons
Great benchtop workhorse
Sample reformatting and cell plating
Extremely easy to use and you can't beat the price. I wish it had a 96 channel head or a gripper so we could expand to a bit higher throughput applications, but overall is a workhorse and does exactly what we need.
Review Date: 27 Mar 2023 | Opentrons
The $4000 Opentrons OT-2 liquid handling robot was designed specifically for high-throughput life science labs that haven't historically considered automation, which can cost 5-20x more than the baseline OT-2. Opentrons is vertically integrated, meaning design, production, service and sales don't rely on 3rd parties or bloated bureaucracies that inflate costs at long-established organizations. Opentrons also focuses on ease of use, open-source hardware/software, and web-based support, all of which allow for a more modern, seamless experience with liquid handling automation, while allowing further savings to be passed to your lab.
Features:
- Price - order online starting at $4000
- Volume - 1-1000µL
- Capacity - 11 microplates & 1 discard bin
- Pipettes - up to two 8-channel
- Accuracy - 1uL ~15%
- Precision - 1uL~ 5%
- Speed - 20 sec. to fill 96-well plate
- Calibration - xyz tip probe calibration
- Replacement Pipette - starting at $500
- Max Run Space - 63cm x 57cm
- Max working height - 37cm
- Connectivity - wifi and/or USB 2.0
- Hood compatibility - coming soon
- Camera - yes
- Mounts - 2 pipette mounts
- Flexibility - open-sourced, customizable, or out-of-the-box
- Support - complimentary support
- Warranty - 1-year
Fully automated hybridization-based targeted sequencing sample prep with superior uniformity in 6 hours
Automation has become increasingly vital to accommodate the growing demands of sample throughput, reliability, and reduced hands-on-time. Automating Avida’s Point-n-Seq® (PnS) hybridization-based targeted sequencing chemistry on the Opentrons® OT-2 liquid handler provides processing efficiency in NGS sample prep library construction and target enrichment, without compromising NGS performance data. Discover how this automated solution greatly accelerates the development of fast, simple, and accurate genomic profiling assays for current and future clinical applications.
Automating complex manual sample preparation for mass spec-based proteomics using the Opentrons OT-2 platform
As quantitative proteomics experiments increase in scale and complexity, greater consistency and precision are required, which is best delivered by automation. Dr. Joao Paulo and his laboratory at the Harvard Medical School Department of Cell Biology employ mass spectrometry-based proteomics (MSP) analysis to better understand the effects of molecular mechanisms and cell signaling pathways on the regulation of the proteome of various model systems. He sought to replace labor-intensive manual sample preparation with automation, and his work serves as a demonstrated case study for successful automation of sample preparation for mass spectrometry-based proteomics using the Opentrons OT-2.
