MicroCal PEAQ-ITC

This application note discusses how isothermal titration calorimetry (ITC) can be used to generate real-time, enzyme kinetics data, comparable to other enzyme assays.

In this application note, discover how to perform enzyme kinetics experiments with a MicroCal isothermal titration calorimetry (ITC) system.

Isothermal titration microcalorimetry (ITC) is an essential tool in drug discovery and the study and regulation of protein interactions. This brochure describes how Malvern MicroCal ITC calorimeters are designed to deliver the exceptional performance and outstanding quality data needed in these application areas.

In this application note, Malvern Panalytical discuss how isothermal titration calorimetry (ITC) can be used to determine the contribution of individual protein residues or modifications to the total binding affinities of complex substrates. Incorporating binding, thermodynamic and stoichiometry data from ITC, structural studies like NMR and X-ray crystallography, and in vivo studies, will ultimately lead to a complete understanding of these complex interactions.

In this application note, Malvern Panalytical discusses how isothermal titration calorimetry (ITC) generates real-time enzyme kinetics data, comparable to other enzyme assays, and explores how to perform enzyme kinetics experiments with a MicroCal ITC system.

In this application note, practical tips are provided for scientists conducting Isothermal titration calorimetry. These tips pertain to things such as:

• The removal of bubbles
• Residual solution in the ITC cell
• Remaining sample solution in a cell reservoir
• Importance of cleaning the cell reservoir and pipette retaining nut
• Washing and rinsing error troubleshooting

Unfolded proteins fold into thermodynamically-favorable native structures under the correct physiological conditions for gain of function. This whitepaper describes how differential scanning calorimetry and isothermal titration calorimetry can be used as a powerful method for the thermodynamic investigation of interactions between diverse biomolecules and inorganic compounds. It details some examples from literature of the methods application in studying amyloid fibrillation.

Isothermal titration calorimetry (ITC) is an analytical technique that has become the “gold standard” for studying intermolecular interactions. As its name indicates, it is a titrimetric technique, that is, a volumetric laboratory method for quantitative chemical analysis (traditionally intended to determine the unknown concentration of an identified analyte) where a reagent solution, the titrant, is made to react with a solution of analyte or titrand. This white paper details the use of ITC in biophysical studies.

Measurements and characterization of binding interactions between proteins and low-molecular weight ligands are fundamental for drug discovery. Among the most recognized challenges in characterizing binding interactions are (1) the need to accurately assess a wide span of binding affinities (KD) and (2) accurately rank and characterize low-molecular weight (LMW) ligands based on affinity, mechanism of action, and energetics of interaction.

Measurement and characterization of binding interactions between proteins and low-molecular weight (LMW) ligands are a focus of academic research and drug discovery. Isothermal titration calorimetry (ITC) directly measures heat released or absorbed in a binding event, providing means for studying protein-small molecule interactions in solution without the need for labeling or immobilization. Importantly, ITC is often utilized to characterize differences in entropic and enthalpic contributions to binding of novel ligands.

This Malvern Panalytical masterclass delves into the intricacies of interaction analysis and explores how ITC and GCI are complementary and orthogonal techniques for all early-stage drug discovery needs. Discover how ITC and GCI technologies can be applied to complex targets and interactions in a series of applications from hit identification to lead-optimization.

Over the last twenty years, Fragment-based drug design (FBDD) has emerged as a prominent strategy. Yet FBDD is not without challenges, including commercial libraries often hampered by excessively sized fragments. Recently new assaying techniques have emerged that push the boundaries of what’s possible with fragment screening, offering collections specifically tailored to biophysical screening. In this webinar, two respected industry authorities reveal how next-generation FBDD fragment screening can assist biophysical researchers more efficiently identify and prioritize the best fragment hits, compress hit-to-lead campaign times, and reduce research cost and raise lead throughput.

This year’s Reviewers’ Choice Award for Analytical Science Customer Service of the Year went to Malvern Panalytical. In this video, Dr. Anne Virden, Product Manager Micro Materials at Malvern Panalytical, highlights the value of user reviews. Virden explains how customers are always at the center of what the company does and how product reviews on SelectScience enable Malvern Panalytical to truly understand their customers’ needs.

Take a look at the reviews left by scientists around the world for Malvern Panalytical’s products and leave your own reviews on the lab equipment you use.

Learn more about the MicroCal PEAQ-ITC, the most advanced Isothermal Titration Calorimetry system from Malvern Instruments, which offers high quality data, ease of use, and exceptional sensitivity. Suited to life sciences and drug discovery applications, this microcalorimetry instrument is perfect for researchers wishing to study biomolecular interactions.

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