Mastercycler® Services

Name: Mastercycler® Services
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Amplify your performance To ensure your instrument is operating consistently at peak performance, we have developed service programs and options to meet your needs. Enjoy peace of mind with Eppendorf Performance Plans and Services for your Mastercycler pro, Mastercycler ep and Mastercycler family thermal cyclers. Performance Plans Features 1. Eppendorf service quality and integrity 2. Certified reports 3. Customized service op…

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Amplify your performance

To ensure your instrument is operating consistently at peak performance, we have developed service programs and options to meet your needs. Enjoy peace of mind with Eppendorf Performance Plans and Services for your Mastercycler pro, Mastercycler ep and Mastercycler family thermal cyclers.

Performance Plans Features

1. Eppendorf service quality and integrity

2. Certified reports

3. Customized service options

Benefits of Preventive Maintenance

1. Highest confidence in results

2. Permanently reliable and reproducible results

3. GLP compliant audited systems

Performance Plans are available in selected countries only.

Application NoteLife Sciences

Automated Illumina® TruSeq® Stranded Total RNA library construction with the epMotion® 5075t/TMX

This application note describes the configuration and preprogrammed methods for the automated construction of 8/16 or 24 sequencing ready libraries from 100-1000ng total RNA using the Illumina TruSeq Stranded Total RNA kit. The overall hands on time is less than 1 hour, the total run time of the entire procedure is ~11.5 hours for 24 samples.

29 Jun 2015

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Mastercycler® Services

Eppendorf

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Eppendorf PCR tubes
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Original Eppendorf PCR tubes are manufactured to the highest Eppendorf quality standards. These thin-walled polypropylene tubes ensure efficient and homogeneous heat transfer in the sample thanks to the even wall thickness and smooth wall surface. Heat-sealed bags enclosed in boxes sealed with a PCR Clean sticker ensure the highest degree of purity. A frosted labeling area on the tube allows easy sample tracking
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Eppendorf PCR-Cooler
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Product features Pink or light blue above 7°C, violet or dark blue when ice-cold DescriptionThe new Eppendorf PCR-Cooler maintains a sample temperature of 0°C*1 for over an hour and then changes color when the temperature has exceeded 7°C. Superior to an ice bath, PCR-Cooler is easy to handle, space-saving and free of contamination, which makes it the perfect preparation for your PCR reactions. The temperature of single tubes is indicated by the color of the individual bores. Further, samples are prevented from freezing by the specially developed cone contour. Eppendorf PCR-Cooler - the cooling aggregate in the universal 96-well format for 0.2 ml and 0.5 ml PCR tubes, for tubes strips, plates and individual tubes. For a compact, innovative solution for protecting, transporting and storing sensitive samples as well as for safely stopping reactions, you need look no further than Eppendorf PCR-Cooler. *1 With 2 hours' pre-cooling at -20°C.
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Mastercycler® Series
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Eppendorf Mastercycler is the economical option when, after having optimized your PCR using Mastercycler gradient, you wish to carry out an experiment with the usual Mastercycler quality, but without blocking your optimization cycler.The entire Eppendorf Mastercycler family boasts the personal card system. Chip-based memory cards enable not only simple and reliable transfer of your individual protocols between different Mastercycler workstations but also ensure unlimited expansion of the memory capacity.The universal block for different tube types extends the applicational range of Mastercycler and Mastercycler gradient as well as saving time and money. It is possible to use 96 x 0.2 ml tubes, 77 x 0.5 ml tubes or one microtiter plate - without having to change the block. Based on its recent analysis of the PCR instrumentation market, Frost & Sullivan recognizes Eppendorf with the 2012 European Green Excellence Award for its Mastercycler nexus. Mastercycler® Series Features: Heating rate: 5°C/s Fast silver block Small footprint Intuitive graphic programming Up to two other units can be connected to a central unit E-mail notification Flexlid concept: automatic height adjustment of the lid allows you to use all types of consumables 2-year warranty Optional self-test functionality Mastercycler® Series Applications: Fast PCR Standard PCR Cycle sequencing
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Temperature-Validation System
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The Temperature-Validation System for the entire Mastercycler family enables simple and rapid validation and calibration of the temperature on the universal blocks of the cyclers. The program for automatic measurement and calibration is integrated into the Mastercycler software, thus furnishing the Mastercycler family with an accurate and precise system that meets all documentation and detection requirements in the laboratory.
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CycleManager
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The Eppendorf CycleManager is a Windows based software for programming and controlling one or more Mastercyclers. The thourough databank ensures reliable storage of all GLP relevant experimental data incl. detailed user comments.The main dialogue box is the central user interface. All key functions can be accessed from it. Mastercyclers can be added or removed from the Multi-Cycler-Network. It offers also an overview of all connected cyclers.
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0.2 and 0.5 ml PCR tubes
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Original Eppendorf PCR tubes are manufactured to the highest Eppendorf quality standards. These thin-walled polypropylene tubes ensure efficient and homogeneous heat transfer in the sample thanks to the even wall thickness and smooth wall surface. Heat-sealed bags enclosed in boxes sealed with a PCR Clean sticker ensure the highest degree of purity.
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Cap Strips
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• Strips with 8 microcaps for 0.2 ml wells• Easy and rapid sealing of Eppendorf twin.tec plates and other standard microtiter plates, see also Capping Aid• Easy to remove using extension at the end of the strip• Autoclavable (121°C, 20 min.)• Certified free of human DNA, DNase, RNase and PCR Inhibitor**Certificate, test procedures and detailed information on request.
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twin.tec PCR Plate 96 and 384
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Eppendorf® twin.tec PCR plates give you the advantages that come from combining two materials: the wells are manufactured from virgin polypropylene, the most successful material used for PCR tubes; polycarbonate, an especially rigid material, forms the plate deck and the skirt. This one-piece design is achieved by an innovative injection moulding process, twin-shot technology. Polycarbonate enhances the mechanical stability and facilitates handling for the twin.tec PCR plates. Its enhanced stiffness guarantees improved plane parallelism in the plate deck before, during and after PCR, thus ensuring a more secure seal. Minimum sagging of the skirt makes the plate the ideal choice for use in automation and in conjunction with robots. All these features are nevertheless of equal use to molecular biologists working in manual operation, because the visible outcome is a batch of more reliable PCR results. Polypropylene gives the wells an inert, non-wetting surface that prevents DNA, RNA and enzymes from binding and improves recovery even when you are working with the most minute volumes. Compared to the common thin-walled tubes, Eppendorf twin.tec PCR plates offer a further 20% reduction in wall thickness. This provides an ideal snug fit for the well against the thermal block and ensures virtually undisturbed heat transfer from the block to the sample. Raised well rims optimize sealing effectivity and reduce the risk of cross contamination. All commonly used sealing methods can be applied: cap strips, adhesive films and foils - and for best protection against evaporation, heat sealing.Eppendorf's certified purity standard - "PCR clean" - guarantees that the product is free of human DNA, DNase, RNase and PCR inhibitors.
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Deepwell Plates 96, 1.2 ml and 2.2 ml
Eppendorf
Ideal plate for:Cell culture Reactions in 96 Well format up to 1,2 ml i.e. 2,2 ml Storage of samples and transport Round base facilitatesrecovery of samples Compatible with automated systems Can be used with adhesive seal, heat sealing or with theautoclavable Deepwell Mat 96 Autoclavable (121°C, 20 min.)
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PCR Film, adhesive, and PCR Foil, adhesive
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Effective adhesive seal prevents evaporation during reaction and storage Can be removed from plate without leaving any residue Autoclavable (121°C, 20 min.) Certified free of human DNA, DNase, RNase and PCR Inhibitor*1 PCR Film, adhesive Sample can be visually monitored through the transparent film PCR Foil, adhesive Easy to pierce Pipette tip does not become tacky (ideal for automated systems) *1 Certificate, test procedures and detailed information on request.
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PCR Rack
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Rack for handling and storing tubes and plates For 0.2 ml, 0.3 ml, 0.5 ml tubes and 96-well microtiter plates Stackable Can be stored down to -90°C Autoclavable (121°C, 20 min.)
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For all workstations, additional microscopes, adapters, video equipment and application-specific accessories may be required.
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UVette®
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UVette®

Eppendorf

The UVette fulfils the highest specifications of modern spectrophotometric analysis in the UV range and can be optimally used for sensitive samples and small volumes. Contamination-free!Individually packaged, UVettes are DNA, RNase and protein-free, and are protected from scratches and dust: These are ideal prerequisites for reliable results and safe transport in the lab. Safe and transparent!The plastic material of the UVette provides a transparency range of 220-1,600 nm. It is possible to carry out measurements in the UV range as well as the entire VIS range. Pipetting can be monitored clearly through the crystal-clear plastic and is always completely bubble-free, even for the minute 50 µl sample volume. The design of the cuvette has a funnel-shaped base, which prevents the capillary effect, and ensures that the liquids constantly remain in the center of the measuring area.A perfect design!Two optical path lengths with a single rotation! Samples with a normal concentration can be measured using the 10 mm optical path length. For samples with a higher concentration, simply rotate the UVette by 90° and use the shorter 2 mm optical path length. The use of quartz glass cuvettes can frequently compromise measuring results due to contamination or mechanical damage in the repeated rinsing process. The careful design of the UVette with its inwardly facing optical windows provides vastly superior protection against scratches when inserted into the photometer.Universal!The new UVette is ideal for use with the Eppendorf BioPhotometer. In other photometers or spectrometers the Uvette is inserted with the aid of an adapter. This adapter aligns the UVette to the corresponding height of the optical light path and also serves as an aperture for optimal light guidance and use of the small measuring volume.Precise!Our vast experience in the manufacturing of optical precision parts is evident in every detail. The UVette is subject to our rigorous quality control and test procedures, which safeguard the high-quality standards for which Eppendorf is renowned throughout the world.

(1)

Automated Illumina® TruSeq® Stranded Total RNA library construction with the epMotion® 5075t/TMX

Product Overview

Mastercycler® Services

Links

Featured products

Bioprocess Autosampler

twin.tec® Trace PCR Plates

Eppendorf PCR tubes

Eppendorf PCR-Cooler

Mastercycler® Series

Temperature-Validation System

CycleManager

0.2 and 0.5 ml PCR tubes

Cap Strips

twin.tec PCR Plate 96 and 384

Deepwell Plates 96, 1.2 ml and 2.2 ml

PCR Film, adhesive, and PCR Foil, adhesive

PCR Rack

Microscope Adapters

UVette®

Bioprocess Autosampler

twin.tec® Trace PCR Plates

Eppendorf PCR tubes

Eppendorf PCR-Cooler

Mastercycler® Series

Temperature-Validation System

CycleManager

0.2 and 0.5 ml PCR tubes

Cap Strips

twin.tec PCR Plate 96 and 384

Deepwell Plates 96, 1.2 ml and 2.2 ml

PCR Film, adhesive, and PCR Foil, adhesive

PCR Rack

Microscope Adapters

UVette®