Human Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are a relatively rare cell population, and large quantities of cells are typically required for clinical applications. This creates the need for large-scale platforms to efficiently expand MSCs. MSCs have gained significant attention because of their therapeutic potential for many difficult-to-treat diseases. In this application note, PromoCell describes an easy-to-understand protocol for the Good Manufacturing Practice (GMP)-compliant large-scale production of MSCs in a single-use bioreactor. The note also includes results regarding attachment and cell expansion, and results of the metabolite analysis.

Mesenchymal stem cells (MSC) are fibroblastoid multipotent adult stem cells with a high capacity for self-renewal. So far, these cells have been isolated from several human tissues, including bone marrow, adipose tissue, umbilical cord matrix, tendon, lung, and the periosteum. Recently it has been shown that MSC originate from the perivascular niche, a tight network present throughout the vasculature of the whole body. These perivascular cells lack endothelial and hematopoietic markers, e.g. CD31, CD34 and CD45, but express CD146, PDGF-R beta, and alkaline phosphatase.

Mesenchymal stem cells (MSCs) are fibroblastoid multipotent adult stem cells with a high capacity for self-renewal, and have been isolated from a variety of human tissues. This poster defines three criteria developed by the International Society for Cellular Therapy (ISCT) for ensuring the integrity and identification of human MSCs, providing a common set of comparable criteria for MSC research.

For science to move forwards, the research we do must be reproducible. One of the ways PromoCell is working to help your research be as consistent as possible is through its characterized MSCs. In this video, find out how the organization ensures its MSCs are characterized.