Centro LB 963 Microplate Luminometer
ULTRASENSITIVE, RELIABLE AND ACCURATE LUMINESCENCE MEASUREMENT
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The Centro is a high-performance, easy to use microplate luminometer for both, flash and glow luminescence applications. The optimized optical system provides lowest background and negligible crosstalk (10-6). Due to its superior sensitivity (XS version: < 1.8 zmol firefly luciferase) and wide dynam ic range (> 6 orders of magnitude), the Centro is ideally suited for all luminescent reporter gene assays, immunoassays (LIA, ILMA), cell-based and biochemical assays.
Centro benefits at a glance:
Have confidence in your results: Ultrasensitive design, excellent crosstalk reduction (10-6) and broad dynamic range (> 6 orders of magnitude) for superior performance.
- Designed to support your research: A built-in shaker, JET-injectors and temperature control (model-dependent) in combination with an ergonomic design and automation compatibility for maximum flexibility.
- Easy setup and analysis: The system is ready to start within minutes (plug & play) and the easy-to-use ICE Software manages instrument control, data collection and data analysis.
- Easy validation: Installation Qualification, Operation Qualification and Performance Qualification steps are performed according to detailed instructions provided (optional 3Q package).
Applications:
- Reporter gene assays
- Aequorin-based calcium monitoring
- ATP determination
- Luminescent Immunoassays (LIA, ILMA)
- DNA probe assays
- SNP determination
- CALUX® Assays
Top tips and tricks: Performing assays in microplates
Microplate assays are now a standard analytical tool in many life science experiments, matched by the corresponding growth of the types of microplate readers available to conduct critical measurements.
In this expert guide, we explore a selection of microplate readers from Berthold Technologies through different assay case studies, explain the basics of assay measurements, and outline common pitfalls. This eBook will also reveal how choosing the right microplate reader for a given assay is vital to overall assay success.
This guide will also outline:
- How to choose the best reader using either filters or monochromators
- How to configure your reader with temperature control
- How to effectively measure flash luminescence
Detection of parasitic molecular strategies to influence host plant signaling
Parasitic plants of the genus Cuscuta are obligate holoparasites with a broad host spectrum for nearly all dicotyledonous plants. As leafless and rootless plants, Cuscuta spp. wind around stems of host plants and penetrate host tissue with haustoria. They directly connect to the vasculature and withdraw water, nutrients, and carbohydrates. Thus, the haustorium development and the establishment of a connection to the host represent essential steps in the parasite’s life cycle.
Molecular cues might be necessary to influence intracellular processes and the connection to the host vascular system. To identify such molecular cues, promotors of host genes that are upregulated at the Cuscuta infection site can be fused to a luciferase reporter gene.
In this webinar, Max Koerner, researcher at FAU Erlangen-Nuernberg, and Francesc Felipe, application scientist at Berthold Technologies, will explore parasitic plant interactions, and discuss how this Promoter: luciferase based bio assays has been used to screen different haustorial Cuscuta-extract preparations for bioactive Cuscuta derived molecular cues.
Key learning objectives
- Understand Parasitic plant interactions in more detail
- Gain insights into Promoter: luciferase based bio assays
- Discover how light emission is used as readout
- Learn about the newest generation of Microplate Readers that help you master your daily research
Who should attend
The content of this event is ideal for researchers, professors, and scientists that are performing Luciferase-based assays to study gene regulation and are looking for application flexibility to master changing plate reader applications.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
