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Synergy and Cytation Readers Validated for Agilent MitoXpress and pH-Xtra Assays

Agilent Technologies
25 Jun 2019
Georgina Wynne Hughes
Editorial Assistant

Product news

BioTek is pleased to announce that its Synergy™ H1 and Synergy Neo2 Hybrid Multi-Mode Readers and its Cytation™ 1 and Cytation 5 Cell Imaging Multi-Mode Readers are validated for use with the MitoXpress and pH-Xtra Assays from Agilent Technologies. The assays may be used alone or combined to measure metabolism in live cells. Instructional Applications Bulletins are available for each multi-mode reader detailing the specific parameters for optimized TRF performance with MitoXpress Xtra Oxygen Consumption, MitoXpress Intracellular Oxygen and pH-Xtra Glycolysis products.

To date, BioTek’s Cytation 1 and Cytation 5 are the only automated imagers approved for Agilent Seahorse XF data normalization; generating reliable cell counts for improved assay reproducibility and more meaningful biological connections between cell seeding conditions and XF data. Cytation instruments combine automated digital microscopy with filter- and monochromator-based reading for a wide variety of quantitative and qualitative measurements in cell-based assays and more. Advanced Synergy Neo2 and basic Synergy H1 Hybrid Multi-Mode Readers offer highly flexible microplate-based detection through combined filter- and monochromator-based reading.

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Agilent BioTek Synergy Neo2 Hybrid Multimode Reader

Agilent Technologies

Agilent BioTek Synergy Neo2 multimode microplate reader features proprietary Hybrid Technology, with independent optical paths that ensure excellent performance in all detection modes. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF, and up to 4 PMTs provide ultrafast measurements.

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Agilent BioTek Synergy H1 Multimode Reader

Agilent Technologies

Agilent BioTek Synergy H1 is a modular multi-mode microplate reader, with monochromator-based optics and filter-based optics. The proprietary Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your laboratory workflows change.

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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.Cell Based AssaysCell-based assays use living cells to measure biological responses to various treatments or stimuli. They are essential for drug discovery, toxicology testing, and understanding cellular mechanisms. These assays are used to assess cell viability, proliferation, gene expression, and other cellular activities. Browse our peer-reviewed product directory to find the best cell-based assay tools and reagents, compare products, read customer reviews, and get pricing directly from manufacturers.

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