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Revolutionizing the Quest for the Perfect Cup of Coffee

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Discover the innovations which have led to an improved method of sugar analysis

28 Dec 2016
Lucy Vass
Administrator / Office Personnel

Editorial article

Jeffrey Rohrer, Ph.D presents a new, faster method for carbohydrate analysis at AOAC 2016

At AOAC 2016 in Dallas, Texas, Jeffrey Rohrer, Ph.D, the Director of Applications Development at Thermo Fisher Scientific, presented a poster entitled ‘A Fast Method for Sugar Analysis for Instant Coffee Samples’. The poster, produced by his colleague Dr Sachin Patil, describes a new high-performance ion exchange chromatography column which can analyze sugars more than ten times quicker than the current AOAC method. In an interview with SelectScience®, Dr Rohrer explains how this upgraded column can be combined with a new system to increase the efficiency of carbohydrate analysis.

Dr Rohrer begins by explaining that carbohydrates in coffee are one of the major dictators of the flavor profile. Therefore, determination of the sugars in coffee samples, including instant coffee and green coffee, is a good method to assess the quality of the product.

He goes on to describe how the current AOAC method for determination of carbohydrates in coffee, which was first developed in 1995, is still used worldwide today. However, this method has the major drawback of being very time consuming as the analysis can take up to 70 minutes.

The new column, the Thermo Scientific™ Dionex™ CarboPac™ SA10, can be used to carry out the same analysis in just six minutes. Dr Rohrer describes how the new column can be used in combination with other innovations to save hands-on time. The new column can be used with the Thermo Scientific™ Dionex™ Integrion™ HPIC™ System, which allows online creation of the mobile phase. Another advantage of the new method is the use of pulsed amperometric detection, meaning the sugars do not need to be derivatized.

In conclusion, the combination of new technologies from Thermo Fisher Scientific have allowed the development of a method that is much more efficient. Ultimately, this method will make it easier than ever before for beverage analysts to assess the quality of coffee samples and improve the quality of the product delivered to the consumer.

Watch the full poster presentation or learn more about the Thermo Scientific™ Dionex™ Integrion™ HPIC™ system.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.AOAC MethodsFood TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.CarbohydratesFood QualityFood quality refers to the attributes of food that influence its safety, nutrition, and taste. Monitoring and ensuring food quality are essential in production to meet health regulations and consumer expectations. Techniques like spectroscopy, chromatography, and microbial testing are used to assess food properties and detect contaminants. Browse our peer-reviewed product directory to find food quality testing solutions; compare products, check reviews, and get pricing directly from manufacturers.

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