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New Sartorius advanced flow cytometry digital handbook

Sartorius Group

A comprehensive and interactive digital guide to advanced flow cytometry with assays, applications, and other resources

31 Oct 2023
Lawrence Howes
Editorial Assistant

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Eileen Hannigan and Sarah Nadin, Assay Development team at Merck
Sartorius Advanced Flow Cytometry Handbook

Sartorius has released its first Advanced Flow Cytometry Handbook as a comprehensive digital guide to assays and workflows on the iQue® Advanced High Throughput Flow Cytometry Platform.

The handbook is designed as a go-to reference for flow cytometry users. Scientists using traditional systems will learn how routine workflows can benefit from the speed, throughput, and multiplexing capabilities offered by the iQue Advanced Flow Cytometry Platform. Labs already familiar with the iQue Platform can stay up to date on new reagents, assay kits, and software releases, as well as the latest Sartorius publications featuring the applications of advanced flow cytometry in both popular and emerging fields.

Similar to other Sartorius’ publications, this handbook includes easy navigation to topics of interest, informative workflow illustrations, detailed application examples, and direct links to external resources for continued learning. Handbook chapters feature iQue Advanced Flow Cytometry applications in cell health, secreted proteins, cell line development, biologics discovery, immune cell models, and cell therapy. Each chapter presents streamlined protocols and validated mix-and-read assays, in addition to publication-ready data figures generated on the integrated iQue Forecyt® software.

The chapter on secreted proteins highlights a unique advantage of the iQue Platform: the ability to combine both cells and beads (Qbeads®) in the same assay, allowing simultaneous assessment of biologically relevant secreted proteins and cytokines. Later chapters demonstrate how this enhanced multiplexing capability translates into richer biological insights in antibody discovery and immune profiling research.

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